Manual

Product Number: EN03

Shipping and Storage

-20℃.

Components

Description

Exonuclease III can gradually cleave single nucleotides along the 3 ‘→5’ direction of double stranded DNA. Each reaction only removes the last few nucleotides to produce progressive deletion of double stranded DNA. This enzyme can cleave double stranded DNA, producing single stranded gaps, but is inactive against single stranded DNA. DNA with flat or 3 ‘recessed ends is usually the most suitable substrate. On the contrary, the 3 ‘protruding end is resistant to cleavage by the enzyme, and the degree of antagonism varies with the length of the 3’ protruding end. Protrusions of four bases or longer cannot be cleaved at all. This characteristic can result in unidirectional deletions in linear DNA molecules with one end being a resistant site (3 ‘protruding end) and the other end being a sensitive site (flat or 5’ protruding end). In addition, compared with BAL 3 Nuclease, this enzyme has lower base specificity. For example, at positions rich in GC, due to the lower probability of reaction stoppage, it can be used for DNA Deletion production.

This product is obtained by expressing the Exonuclease III gene (E. coli) in Escherichia coli and purifying and isolating it multiple times.

Application

1. Site directed mutation;
2. Preparation of chain specific probes;
3. Preparation of single stranded substrates for deoxygenation sequencing.