Manual

Product Number: CK12441H

Characteristics:
Source: HEK293
Description: Human IGF-II(Ala25-Glu91), Accession # P01344.1
Predicted molecular mass: 7.5kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is ≤50 ng/mL.
Formulation: Lyophilized from a 0.2 μm filtered solution containing TFA, ACN, 5% Trehalose.
Size: 10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in 4mM HCl.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.