Manual

Product Number: CK05001

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Recombinant human IL-23 is composed of P40 (Ile23-Ser328) and P19 (Arg23-Pro189) subunits, which are linked by a GS-linker (GSGSSRGGSGSGGSGGGGSKL).
Accession # P29460 (P40), AAG37232 (P19)
Predicted molecular mass:

• 55.0 kDa (IL-23 single chain)
• 36.5 kDa (p40) & 18.5 kDa (p19)

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 μg/mL.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at -20°C to -80°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.