Manual

Product Number: CK04842

Characteristics:

Source: HEK293
Description: Human Vitronectin (Asp20-Leu478)
Accession # P04004
Predicted molecular mass: 53.1 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at -20°C to -80°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.