Manual

Product Number: CK04801L

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Human Vitronectin (Asp20-Leu478)
Accession # P04004
Predicted molecular mass: 52.3 kDa

Specification:
Appearance: Colorless clear liquid
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤1.0 EU/mg by the LAL method
Activity: Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Formulation: 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at -20°C to -80°C under sterile conditions
• 6 months at 2°C to 8°C under sterile conditions
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.