Manual

Product Number: CK04603

Characteristics:

Source: HEK293
Description: Human Wnt-3a (Ser19-Lys352) with a C-terminal linker GGGGGS and Human IgG1 (Asp104-Lys330)
Accession # NP_149122
Predicted molecular mass: 58.2 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤100 EU/mg by the LAL method
Activity: Measured by its ability to induce Topflash reporter activity in CHO-K1 cells. The ED50 for this effect is ＜30 ng/ml.
Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at 2°C to 8°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.