Manual

Product Number: CK04241

Characteristics:
Source: HEK293
Description: Human TGF-β1 (Ala279-Ser390)
Accession #: P01137
Predicted molecular mass: 12.8kDa (monomer)

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.1ng/mL.
Formulation: Lyophilized from a 0.2 um filtered solution containing 100 mM Glycine, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in 4mM HCl.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.