Manual

Product Number: CK01921H

Characteristics:

Source: E.coli
Description: Human IL-1A (Ser113-Ala271)
Accession #: Q53QF9
Predicted molecular mass: 18.0 kDa

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤100 EU/mg by the LAL method
Activity: Measured by its ability to induce NF-kB reporter activity by the reporter gene assay. The ED50 for this effect is ≤0.05 ng/ml.

Formulation: Lyophilized from a 0.2 μm filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH 7.4

Size: 50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at 2°C to 8°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.