Manual

Product Number: CK00601

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Human BMP-7 (Met316-His431)
Accession # P18075
Predicted molecular mass: 13.1 kDa (monomer)

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 μg/ml.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN, 5% mannitol
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in 4 mM HCl.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to -80°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.