T4 UvsX Recombinase
2025-06-03
Product Number: CK001
1. Introduction
This document describes the recommended usage instructions for the Human NK Cell Expansion Kit (Catalog Number CK001) for NK cell expansion. The kit contains three lyophilized products: CK001-1A, CK001-2A, and CK001-3A. This kit is designed to be used with an initial culture medium. The specific usage recommendations are as follows:
2. Product Reconstitution
CK001-1A: Dissolve the lyophilized powder in 100 μL of water, then add 7.4 mL of PBS to prepare a coating solution; total volume is 7.5 mL. Store at -20°C or below.
CK001-2A: Dissolve the lyophilized powder in 100 μL of water, then add 9.9 mL of initial culture medium to prepare an activation factor; total volume is 10 mL. Store at -20°C or below.
CK001-3A: Dissolve the lyophilized powder in 270 μL of water, then add 9.73 mL of initial culture medium to prepare an expansion factor; total volume is 10 mL. Store at -20°C or below.
3. Usage Summary Table
| Step | Medium |
| Coating | CK001-1A, 7.5 mL coating solution |
| Activation Medium | 190 mL initial culture medium + 10 mL CK001-2A: Mix the two together for use |
| Expansion Medium | 1800 mL initial culture medium + 10 mL CK001-3A: Mix the two together for use |