BsiWI
2025-05-27BspQI
2025-05-27
Product Number: RE0581
Shipping and Storage
-20℃.
Components
| Component | Specifications V | Specifications S |
| BsmBI(10U/μL) | 20μL | 100μL |
| 10×Cut Buffer C | 1mL | 1mL |
Description
BsmBI belongs to the Type IIs restriction enzyme, which can recognize non palindromic sequences and perform cleavage outside of the recognized sequence. It is commonly used for Golden Gate assembly. The optimized reaction buffer maximizes the functionality of BsmBI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.
Suggested reaction conditions
- 1 x HN buffer solution;
- Incubate at 55℃;
- Prepare the reaction system according to the "DNA enzyme digestion process".
Inactivation conditions
Incubate at 80℃ for 20 minutes.