Manual

Product Number: RE0583

Shipping and Storage

-20℃.

Components

Description

BspQI belongs to the Type IIS restriction enzyme, which recognizes non palindromic sequences and performs cleavage outside of the recognized sequence. It is commonly used for Golden Gate assembly. The optimized reaction buffer maximizes the functionality of BspQI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.

Suggested reaction conditions

1. 1×HN buffer solution;
2. Incubate at 50℃;
3. Prepare the reaction system according to the “DNA enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.