A Guide to the Selection of Methods and Products for MicroRNA RT PCR
There are two common principles and methods for microRNA reverse transcription/microRNA fluorescence quantitative detection:
- PolyA tail addition method (most commonly used)
- Stem loop
The first method: PolyA tail addition
To complete the PolyA tail method, it actually requires two components. One is a microRNA reverse transcription kit, and the other is a microRNA fluorescence quantitative detection kit. The biggest feature of the PolyA tail method is that the primers used by each company are confidential and different, so each company's polyA tail method microRNA reverse transcription kit and matching microRNA fluorescence quantitative detection kit must be used together. If you use Company A's microRNA reverse transcription kit, you must use Company A's matching microRNA fluorescence quantitative detection kit. If you use Company A's microRNA reverse transcription kit but use Company B's fluorescence quantitative detection kit, the experiment may fail. Of course, there is an exception, which is that when Company A is making a microRNA reverse transcription test kit, it has already provided its own company with special primers for the microRNA fluorescence quantitative detection kit. Therefore, it is not necessary to buy Company A's microRNA fluorescence quantitative detection kit for use, but to buy any company's fluorescence quantitative mix and match it with this special primer.
PolyA Tail Method Product Introduction:
PCK50 Enhanced microRNA First Chain Synthesis Kit
PCK51 enhanced microRNA fluorescence quantitative PCR detection kit (consisting of fluorescence quantitative mix and special matching primers)
PCK50+PCK51 must be used together. If you buy PCK50, you will definitely need to buy PCK51 to use it together.
If you want to only buy PCK50 for microRNA reverse transcription and do not want to buy the matching PCK51, downstream fluorescence quantitative detection using other companies' fluorescence quantitative mix may not be able to complete downstream experiments due to primer mismatch.
PCK52 Enhanced microRNA Reverse Transcription/Fluorescence Quantitative Detection Kit (All In One)
PCK52=PCK50+4 PCK51. Generally speaking, microRNA reverse transcription is used less and microRNA detection is used more. This ratio arrangement is suitable for practical situations. At the same time, it can be made into a two in one kit for users to purchase together, which can be cheaper than purchasing separately.
Second type: Stem loop
Let's talk about the second principle of microRNA reverse transcription, the stem loop method microRNA reverse transcription. Its biggest feature is that the microRNA reverse transcription kit does not need to be matched with a specific microRNA detection kit. As long as you purchase the stem loop method microRNA reverse transcription kit, it can be used with any company's ordinary fluorescence quantitative mix.
Product Introduction of Stem Ring Method
PCK53-MRNA Stem Loop cDNA Kit Stem Loop microRNA Reverse Transcription Kit
