Product Number: RNK35H200

Shipping and Storage

-20℃

Components

Description

This product is a recombinant murine RNase inhibitor expressed and purified in Escherichia coli, with a molecular weight of approximately 50 kD. It can specifically inhibit the activity of RNase A, B, and C,Can form a 1:1 complex with RNase, thereby inhibiting its activity. This reaction is reversible, as urea and thiol reagents can dissociate the complex, causing RNase to be renaturated while inhibitors are irreversibly deactivated. When in use, it can be directly added to the reaction solution containing RNA. This product belongs to protein properties and is different from other competitive inhibitors (nucleic acids, inorganic phosphates). It can be easily removed from the reaction system by phenol treatment.

Compared with human RNase inhibitors, murine RNase inhibitors have higher antioxidant activity and are more suitable for experiments with high DTT sensitivity.

This product does not contain ingredients such as glycerol that affect the freeze-drying process, and can be used for the preparation of freeze-drying reaction systems and product design.

Application

This product can be used in any experiment that requires avoidance of RNase interference to prevent RNA degradation, such as:

1. CDNA synthesis reaction.
2. External translation.
3. Polyribosome separation.
4. There is no cellular system transcription in vitro.

Unit definition

The amount of enzyme required to inhibit 50% of 5ng RNase A activity is defined as one activity unit (U). The activity of RNase A was quantitatively determined by inhibiting the hydrolysis of Cyclic2 ‘, 3’ – CMP to generate 3 ‘- CMP

Quality control

After multiple column purifications, SDS-PAGE gel detection showed only a clear and single target band; The qPCR method detects no residual E. coli DNA and no contamination of nucleic acid endonucleases.

Manual

Product Number: BS02

Shipping and Storage

-20°C.

Components

Description

WellStart II BST DNA polymerase is a recombinant enzyme expressed and purified by Escherichia coli. Its gene is derived from Bacillus stearothermophilus and has undergone partial point mutations based on the original sequence.This protein has stronger 5’→ 3′ DNA polymerase activity, chain displacement activity, reverse transcriptase activity, and no 5’→ 3′ exonuclease activity.Applied to DNA/RNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), etc.

Unit definition

The amount of enzyme required to add 10nmol of deoxynucleotides to acidic insoluble substances within 30 minutes at 65℃ is defined as 1 active unit (U).

Heat Inactivation

Incubate at 80°C for 5 minutes before inactivation.

Quality Control

After multiple column purification, the purity was detected by SDS-PAGE to be greater than 98%; No exogenous nuclease activity was detected.

Test

No activity at room temperature. Will be activated at 60°C.

Product Number: RN01

Shipping and Storage

-20°C.

Components

Description

RNase H, Recombinant is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA.This product can be used for the detection of DNA-RNA hybrids, removing mRNA during the synthesis of the second strand of cDNA, and removing the poly (A) tail of mRNA hybridized to poly (dT).

Features

This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70°C or above for 10 minutes.

Unit definition

Using poly (rA)·poly (dT) as the substrate, the enzyme required to produce 1nmol of acid soluble substance within 20 minutes at 37°C is defined as 1 active unit (U)

Storage Buffer

Tris-HCl (pH7.5) 25mM，NaCl 30mM，DTT 1mM，EDTA 0.5mM，Glycerol 50%.

Single-Stranded DNA Binding Protein, Thermostable SSBP, Increase the yield and specificitiy of PCR

Description

Thermo stable Single-Stranded DNA Binding Protein is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95°C for 60 minutes. Due to the extreme thermostability, thermaostable SSBP can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

Applications

RT-PCR & cDNA Synthesis

Product Source

An E. coli strain that carries the cloned ssb gene from a hyperthermophilic organism

Features

• Improve the processivity of DNA polymerase
• Stabilization and marking of ssDNA structure
• Increase the yield and specificitiy of PCR
• Increase the yield and processivity of RT during RT-PCR
• Improve DNA sequencing through regions with strong secondary structure

Transportation

on blue ice

Storage

at -20°C for 24 months

Order

Product Number: UG01

Shipping and Storage

-20°C.

Components

Description

Uracil-N-glycosylase (UNGase), also known as uracil DNA glycosylase (UDGase), is a recombinant enzyme expressed and purified by E. coli. The protein has a molecular weight of 25 kD and can catalyze uracil-containing Single- and double-stranded DNA releases free uracil and is inactive to RNA, and is mainly used to prevent contamination of PCR amplification products. The mechanism of action of the enzyme is as follows: in the PCR reaction, dUTP is used instead of dTTP, and all Ts in the amplified product fragments are replaced by U, forming a PCR amplification product containing dU bases. UNG enzyme can selectively break the glycosidic bond of U base in single-stranded and double-stranded DNA, degrade the DNA containing U in the reaction system, effectively eliminate the residual contamination of PCR products, and greatly reduce false positives caused by contamination of amplification products, thereby ensuring specificity and accuracy of amplification.

Activity Definition

One unit is defined as the amount of enzyme required to catalyze 1nmol of uracil from uracil-containing DNA in 60 minutes at 37°C.

Quality Control

The purity detected by SDS-PAGE was more than 95%; no endonuclease or exonuclease activity was detected

Description

Taq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus. The molecular weight of the product is approximately 94 kDa. EasyTaq DNA polymerase has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′ -5′ exonuclease activity. The extending speed is 1-2 kb/min. There is an “A” on 3′ end. The PCR product can be cloned in TA vector.

• CAS No. : 9012-90-2
• Other Names: nonstandard Taq DNA Polymerase
• Purity: 99.9%
• Classification: Specific Reagents
• nonstandard Taq DNA Polymerase: High concentration 100Unit

Package Details

• small package or bulk package both OK. Ship with blue ice and form box.
• storage buffer without glycerin.
• Offer OEM ODM and bulk service!
• high-sensitivity, high amplification Taq DNA polymerase. offer to all over the world, supply Bulk and OEM.

Characteristics

(1) high-sensitivity
(2) high amplification efficiency

Unit Definition

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Quality Control

• functional absence of double- and single-stranded endonuclease activity;
• Purity>90% homogeneous by SDS gel electrophoresis.
• Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

Order

Product Number: TR02

Shipping and Storage  

-20℃

Components

Description

Thermalstable T7 RNA Polymerase is a genetically modified T7 RNA polymerase.This enzyme highly specifically recognizes the T7 promoter sequence and can perform efficient in vitro transcription at 50℃.During the in vitro transcription process, it can improve the capping efficiency and eliminate the production of dsRNA byproducts.

Features

The T7 promoter has high specificity and is used for the synthesis of in vitro RNA (including small RNAs).

Application

1. Synthesis of single stranded RNA;
2. Synthesis of highly specific RNA probes;
3. Synthesis of siRNA precursors;
4. Produce precursors for RNA splicing reactions;
5. Using cap analog as a primer, produce Capped mRNA.

Unit definition

The amount of enzyme required to mix 1 nmol of [³H] GMP with acid insoluble precipitate within 1 hour at 50℃ and pH 8.0 is defined as 1 active unit.

Quality Control

After multiple column purification, SDS-PAGE gel detection only showed clear and single target bands, with a purity of 95%. PCR detection showed no residual Escherichia coli DNA and no contamination of RNase, nucleic acid endonucleases, and exonucleases.

Suggestions

1. For effective transcription in specific regions, it is recommended to pre cut the template DNA into flat or 5 ‘protruding ends downstream of the region.
2. The binding of spermidine in the buffer with nucleic acid may form insoluble substances, and it is recommended to add template DNA finally.

Related Products

TR01 – T7 RNA Polymerase

TR02 – Thermostable T7 RNA Polymerase

TR03 – T7 RNA Polymerase 200U/ul

GMP-T701 – T7 RNA Polymerase, GMP Grade

E131 – T7 High Yield RNA Transcription kit

Description

CSM Taq polymerases (Cold sensitive mutant Taq polymerases ) is a kind of HotStar Polymerase. Source from the mutant E. coli. CSM Taq polymerase unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq, Cold Taq is the cold sensitive mutant Taq. It retain the hot start ability throughout the whole amplification cycles.

Cold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites.

Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.

Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.

Cold sensitive mutant Taq polymerases are capable of extending through difficult regions, e. g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.

Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.

Applications

• Hot start PCR amplification
• Specific amplification of complex cDNA and genomic template, for amplification of difficult templates, such as GC-rich fragments and microsatellites
• Primer extension of SNP markers
• Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity
• Amplification from low copy number DNA template, high through-put Hot Start PCR with high specificity, sensitivity, and yield
• Routine diagnostic Hot Start PCR requiring high reproducibility.
• Real-Time PCR
• Multiple PCR
• Generation of PCR products for TA cloning

Quality Control

• Functional absence of double and single-stranded endonuclease activity;
• Purity>99% test by SDS gel electrophoresis;
• Each lot of CSM Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Concentration: 5 U/μl

Storage: Store at -20°C

Package: Bulk

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This product is a GMP grade recombinant Pyrophosphatase, Inorganic (yeast) (PPase, yeast). It can hydrolyze the inorganic pyrophosphatase produced in nucleic acid amplification experiment, promote the reaction balance to shift to the end of product generation, and increase the amount of product.

This product is a recombinant inorganic pyrophosphatase expressed on a large scale in E. coli.Its molecular weight is about 63kd, which conforms to GMP production and quality management standards. All raw and auxiliary materials are traceable.

Specification

• No virus, mycoplasma and other common pathogens
• Heavy metal: ≤ 10 ppm
• Volume activity (containing cCMP / RNase A): ≥ 600 U / mi
• Internal toxicity control: ≤ 10eu / mg
• Host protein residue: ≤ 50 ppm
• No exonuclease, endonuclease and protease
• Host DNA residue: ≤ 100 pg / mg
• No RNase activity, cleavage activity

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This product is a GMP grade recombinant E. coli poly (a) polymerase, which can catalyze the incorporation of ATP into the 3′ end of RNA in the form of AMP to form a poly (a) tail with 20-200 a bases.
This product is a large-scale expression of recombinant poly (a) polymerase in E. coli, which conforms to GMP production and quality management specifications. All raw and auxiliary materials are traceable.

Specification

• Activity: ≥ 50 Ku / ml
• Purity: ≥ 95%
• Heavy metal residue: ≤ 10 ppm
• No common pathogens
• host protein residue: ≤ 50 ppm
• No residual RNase
• DNA residue in host: ≤ 100 pg / mg
• No residual endonuclease
• Endotoxin content: ≤ 10 EU / mg
• No nonspecific nuclease residue.
• Using RNA as template, add 5u enzyme and add 200 a bases in 10min.
• The poly (a) tail can be added efficiently by adding Tinzyme reagent into the reaction system.

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