2x SYBR qPCR Master Mix no rox

Description

2x Ultra SYBR Mixture (without ROX) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR which include HotStar Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes and Mg²⁺. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes. The chemically modified HotStar Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity.

It can be applied to all qPCR instruments applying ROX as calibration dye. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or the low copy template.

Package

1ml or Bulk

Application

Gene expression analysis
Gene copy number analysis

Features

High sensitivity: exactly quantitate the low copy template
High specificity: maximatily reduce the primer dimers and non-specific product
Wide linear range; Accurate quantitation.

Shipping And Storage

Store in dark at -20°C for up to 1 year.
For frequent use, Store at 2-8°C for short-term (1 week), and avoid freeze thawing.

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