2x SYBR qPCR Master Mix no rox
2x TaqMan qPCR Master Mix with Rox
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2x SYBR qPCR Master Mix Low rox

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2x SYBR qPCR Master Mix Low rox


2x Ultra SYBR Mixture (With Low ROX) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR which include HotStar Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes. The chemically modified HotStar Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity.

The ROX is a dye molecule that can be used to normalize the well-to well fluorescent fluctuations. It is the low concentration in this kit. This kit applied to ABI Prism 7500/7500 Fast, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 and other low rox level PCR instructions.


1ml or Bulk


Gene expression analysis
Gene copy number analysis


High sensitivity: exactly quantitate the low copy template
High specificity: maximatily reduce the primer dimers and non-specific product
Wide linear range; Accurate quantitation.


PCM33L2x Ultra Sybr qPCR Mix (Low ROX)SYRB qPCR Mix, Hotstar tech, with ROX I(Low ROX)
PCM33H2x Ultra Sybr qPCR Mix (High ROX)SYRB qPCR Mix, Hotstar tech, with ROX II(High ROX)

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