ThermoStartTM Bst DNA Polymerase
Description
ThermoStartTM Bst DNA polymerase source from Bacillus stearothermophilus DNA polymerase I. it has no 5'-3' exonuclease activity, but enhanced its 5'-3' DNA polymerase activity and strand replacement activity. After genetic engineering, the enzyme has increased the hot start function, which has no activity below 60 degrees. Compared with wild-type Bst DNA polymerase, the enzyme has greatly improved in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It is very suitable for pollution-proof isothermal amplification reactions, such as lamp.
Source
Recombinant E. coli.
Applications
Isothermal DNA amplification and relate research.
Supplied with
10xBst Buffer.
Unit definition
Under 65 °C for 30 min, the amount of enzyme required to add 10 nmol of dNTP into acid insoluble precipitate defined as 1 Unit.
Thermal inactivation
85 °C,5 min.
Storage and transportation
Ship with ice pack, Stored at -20 °C.
Reaction Set up
Component | Volume(μL) | Final concentration |
10×Bst Buffer | 2.5 | 1×(contains 8 mM MgSO4) |
dNTP Mix (10 mM each) | 3.5 | 1.4 mM each |
dUTP (100 mM) (optional) | 0.35 | 1.4 mM |
UDGase (1U/μL) (optional) | 1 | 1 U |
Template DNA | 10 ng~1 μg | - |
10×Primers* | 2.5 | - |
Thermo Start DNA Polymerase (8 U/μL) | 0.25~1 | 0.08~0.32 U/μL |
ddH2O | to 25 | - |
Reaction conditions
Temperature | Time | Effect |
25~37 °C | 5~10 min | Degradation of U-containing template (optional) |
65 °C | 30~60 min | Reaction |
85 °C | 5 min | inactivation |
Thermo Start Bst DNA Polymerase test
ThermoStart Bst DNA Polymerase, no activity at room temperature. Will be activated at 60°C.
Order
BS01 | Bst DNA Polymerase | Bst DNA Polymerase, Large Fragment, 8U/μl |
BS02 | ThermoStart BST DNA Polymerase★★★ | ThermoStart BST DNA Polymerase, Hotstar technology, High specificity and sensitivity, suitable for RT lamp and IVD purpose |