ThermoStart^TM Bst DNA Polymerase

Description

ThermoStart^TM Bst DNA polymerase source from Bacillus stearothermophilus DNA polymerase I. it has no 5'-3' exonuclease activity, but enhanced its 5'-3' DNA polymerase activity and strand replacement activity. After genetic engineering, the enzyme has increased the hot start function, which has no activity below 60 degrees. Compared with wild-type Bst DNA polymerase, the enzyme has greatly improved in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It is very suitable for pollution-proof isothermal amplification reactions, such as lamp.

Source

Recombinant E. coli.

Applications

Isothermal DNA amplification and relate research.

Supplied with

10xBst Buffer.

Unit definition

Under 65 °C for 30 min, the amount of enzyme required to add 10 nmol of dNTP into acid insoluble precipitate defined as 1 Unit.

Thermal inactivation

85 °C，5 min.

Storage and transportation

Ship with ice pack, Stored at -20 °C.

Reaction Set up

Component	Volume（μL）	Final concentration
10×Bst Buffer	2.5	1×(contains 8 mM MgSO4)
dNTP Mix (10 mM each)	3.5	1.4 mM each
dUTP (100 mM) (optional)	0.35	1.4 mM
UDGase (1U/μL) (optional)	1	1 U
Template DNA	10 ng~1 μg	-
10×Primers*	2.5	-
Thermo Start DNA Polymerase (8 U/μL)	0.25~1	0.08~0.32 U/μL
ddH2O	to 25	-

Reaction conditions

Temperature	Time	Effect
25~37 °C	5~10 min	Degradation of U-containing template (optional)
65 °C	30~60 min	Reaction
85 °C	5 min	inactivation

Thermo Start Bst DNA Polymerase test

ThermoStart Bst DNA Polymerase, no activity at room temperature. Will be activated at 60°C.

Order

BS01	Bst DNA Polymerase	Bst DNA Polymerase, Large Fragment, 8U/μl
BS02	ThermoStart BST DNA Polymerase★★★	ThermoStart BST DNA Polymerase, Hotstar technology, High specificity and sensitivity, suitable for RT lamp and IVD purpose