MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. The difference between this to the general M-MLV RT is that the capacity to endure the heat is enhanced. It can remain the 100% activity at 50°C, it can also keep more than 80% activity even at 55°C.
Recombination of E. coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.
The first-strand cDNA synthesis; RT-PCR
One unit of MMLV RT catalyzes the incorporation of 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using oligo(dT)12-18-primed poly(A)n as a template.
Component: M-MLV (200U/μl) 5xBuffer (with DTT)
|RT01||M-MLV Reverse Transcriptase(RNase H-)||M-MLV Reverse Transcriptase, RNase H Minus 200U/μl, supplied with 5x Reaction Buffer|
|RT02||M-MLV Reverse Transcriptase(RNase H+)||M-MLV Reverse Transcriptase, wide type, RNase H postive. 200U/μl, supplied with 5x Reaction Buffer. High activity.|
|RT03||Thermal Stable M-MLV Reverse Transcriptase(RNase H-)||Thermal Stable reverse transcriptase, RNase H Minus, reaction temperature up to 55°C, 200U/μl|
|RT04||Thermal Super M-MLV Reverse Transcriptase(RNase H-)||Thermal super stable reverse transcriptase, RNase H Minus, reaction temperature up to 60°C, 200U/μl|
|RT05||HiFi II M-MLV Reverse Transcriptase(RNase H-)||M-MLV reverse transcriptase, RNase H Minus, for IVD use|
|RT05L||HiFi II M-MLV Reverse Transcriptase(RNase H-) Lyophilized Powder||M-MLV reverse transcriptase, RNase H Minus, for IVD use, Lyophilized Powder|
|RTY3||HiFi III M-MLV Reverse Transcriptase(RNase H-)||customized M-MLV for COVID|