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USP grade Trypsin

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USP grade Trypsin source from Swine / Bovine pancreas


Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23300 dalton, and is a single peptide chain composed of 223 amino acid residues. With rigorous specificity, the trypsin specifically acts on the peptide linkage constituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation also reduces or loses gradually. It is easily soluble in the water, but insoluble in trichloromethane, ethanol, ether and glycerin, and the optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated when boiled for a short time; if the salt is added into the hot solution, the protein will precipitate, and the enzyme action of filtrate cannot be seen, and Ca2+ plays the role in protecting and activating the trypsin. The high purity Trypsin is purified by re-crystallization, and then by Ion Exchange chromatography and ultra-filtration.


For cell culture, hydrolyze the proteins between cells and disperse them.

Activity ≥ 2500 USP U/mg

Source: Bovine Pancreas


AppearanceWhite or almost white lyophilized powderUSP40
Solubility500,000USP Trypsin Units is soluble in 10ml of water and in 10ml of saline TS.USP40
Loss on dryingNo more than 5.0%USP40
Residue on IgnitionNo more than 2.5%USP40
Microbial limits:
Pseudomonas aeruginosa
Staphylococcus aureus
ChymotrypsinNo more than 50 USP units/mg powderUSP40
AssayTrypsin ≥ 2500USP units/mg powderUSP40
Descriptionwhite crystalline powderEP8.0
IdentificationA) A purple color develops within 3 minutes
B) No purple color develops with in 3 minutes
Appearance of solutionSolution S is not more opalescent than reference suspension ⅢEP8.0
Absorbance1) 280nm : 13.5 ~ 16.5
2) 250nm : not more than 7.0
HistamineNot more than 1µg/0.2µkatalEP8.0
Loss on dryingNot more than 5%EP8.0
Limit of ChymotrypsinNot more than 1%EP8.0
Microbial limits EP8.0
Total viable aerobic count≤10000cfu/g 
Salmonella Absent 
AssayNot less than 0.5µkatal /mg, calculated on the dried basis.EP8.0


Store in an airtight container, at a temperature of 2°C to 8°C.


P062001Trypsin (USP)Specification Activity:Trypsin>=2500U/mg, Chymotrypsin<=2%; Standard USP43; CAS NO. 9002-07-7

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