animal origin free
BspQI is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli.
Recognition sequence is 5′-GCTCTTCN1/N4-3′. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.
This product is produced in accordance with GMP process requirements and supplied in a liquid format.
Recombinant E. coli with BspQI gene.
20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol.
10× Digestion Buffer 500mM Tris-HCl, 1M NaCl,100mM MgCl2, 1mg/mL OsrHSA, pH7.9@25℃.
1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system.
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
1. Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
2. Digestion of DNA to obtain specific sticky ends.
50 μL reaction system.
This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.
1）Add the following components in sequence:
|Plasmid DNA||1-2 μg|
|10×Digestion Buffer||5.0 μL|
|BspQI (10 U/μL)||1.0 μL|
|RNase-free ddH2O||Up to 50 μL|
2）Incubate at 50°C 1h.
3）DNA linearization is complete, and subsequent experiments can be performed.
|M062011||BspQI||BspQI (10U/μl), 10xBspQI Reaction Buffer|