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eGFP mRNA (N1-Me-Pseudo UTP)

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M050803 eGFP mRNA (N1-Me-Pseudo UTP)

Product Description

EGFP mRNA (N1 Me Pseudo UTP) encodes a green fluorescent protein, whose maximum excitation/emission light wavelengths are 488nm/509nm respectively. Transfected cells can express enhanced green fluorescence, which can be used as a control to study the transfection and expression of target genes in mammalian cells. This product can effectively reduce the autoimmunity of mRNA in mammalian cells by introducing N1 Me Pseudo UTP to replace natural UTP, Enhance the stability of mRNA while also simulating mature mRNA with a 5 'Cap 1 structure and a 3' poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 'Cap 1 structure and a 3' poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Legend: eGFP mRNA (N1-Me Pseudo UTP) structure, all UTPs replaced with N1-Me Pseudo UTP

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃

Product packaging

eGFP mRNA (N1-Me-Pseudo UTP) (1mg/ml)100ug1mg

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