(Recombinant Ribonuclease A Lyophilized Powder)

Storage temperature: -20℃

Storage temperature: 4℃

Storage temperature: 25℃

Date: Jun. 16, 2025

(Recombinant Ribonuclease A Solution)

Storage temperature: 25℃

Storage temperature: 37℃

Date: May. 14, 2022

Unlocking Precision in Biomanufacturing: The Power of Nicking Endonucleases

In the rapidly evolving landscape of synthetic biology and molecular diagnostics, one molecular tool stands out for its precision: Nicking Endonucleases.

Unlike traditional restriction enzymes that cut both strands of DNA, nicking endonucleases selectively cleave only one strand of the double helix. This “precision scalpel” capability is transforming how we approach complex molecular biology applications.

Key Applications Driving Innovation

• Isothermal Amplification: Serving as the engine in technologies like NEAR and LAMP, nicking enzymes create precise initiation sites to trigger strand-displacement reactions, significantly enhancing the sensitivity and speed of diagnostic assays.
• Genome Engineering & Labeling: By introducing targeted single-strand nicks, these enzymes minimize the risks of Double-Strand Breaks (DSBs), providing a safer and more precise route for delicate gene manipulation.
• DNA Nanotechnology: Acting as vital tools for structural design, they facilitate the construction of sophisticated DNA nanostructures and molecular machines.

At Tinzyme, we understand that the quality of your molecular tools determines the success of your research and product commercialization. We are dedicated to providing high-performance, ultra-pure enzymes engineered for stability and consistency.

Ready to optimize your workflow? Explore our high-purity enzyme solutions designed for the most demanding applications.

#SyntheticBiology #MolecularDiagnostics #Biotech #Tinzyme #NickingEndonucleases #Innovation #LifeSciences

Product Selection Guide

Note: Animal-Source-Free options are available.

Core Enzymes in Molecular Biology

Behind every breakthrough in genetic engineering and molecular diagnostics lies a set of specialized enzymes. These powerful biological catalysts are the backbone of genetic research and modern biotechnology, enabling critical processes from basic DNA replication to recombinant DNA technology and gene expression studies.

The table below breaks down their key functions, biological roles, and common applications in the laboratory:

Small enzymes. Big impact.

Powering the science that shapes our future.

more:

• What is the difference between Exonuclease and Endonuclease?
• Why is Taq Polymerase used in PCR?

In the world of molecular biology, the stability of enzymes is a critical factor that dictates the efficiency of laboratory workflows and the cost-effectiveness of diagnostic kits. Among these enzymes, RNase A is indispensable for its ability to specifically degrade RNA contamination during DNA and protein purification.

What is RNase A and How Does It Work?

RNase A is a highly efficient ribonuclease that targets single-stranded RNA (ssRNA). It works by catalyzing the hydrolysis of the phosphodiester bond between the 5′-ribose of a nucleoside and the phosphate group of the 3′-ribose of an adjacent pyrimidine (Cytosine or Uridine).

This enzymatic reaction produces 2′, 3′-cyclic phosphate intermediates, which are further hydrolyzed into 3′-nucleoside phosphates. Because of this specificity, RNase A is the gold standard for removing RNA residues in plasmid extraction and genomic DNA purification.

The Advantage of Recombinant Production (Animal-Free)

Traditionally, RNase A was extracted from bovine pancreases. However, this method presents two significant drawbacks:

• DNase Contamination: Animal-derived RNase A often contains residual DNase, which can inadvertently degrade your target DNA and reduce yields.
• Safety Concerns: Use of animal-derived components poses risks in regulated environments.

Tinzyme’s Recombinant RNase A is produced using a specialized yeast expression system. This ensures:

• Zero DNase Activity: High-purity enzymes that protect your DNA samples.
• Animal-Free Process: No animal-derived components are used at any stage of production, making it ideal for gene therapy and cell therapy applications.

Redefining Stability: 4 Months at Room Temperature

The biggest challenge for kit manufacturers is the thermolability of enzymes. Most molecular enzymes require cold-chain logistics, which increases transportation costs and complexity.

To solve this, Tinzyme has optimized its RNase A Solution (Cat. No. RA02) for maximum stability. Our long-term testing confirms that this recombinant solution remains fully stable and active for over 4 months at:

Room Temperature (25°C)

Elevated Temperatures (37°C)

Why This Matters for Your Business

• Simplified Logistics: No need for dry ice or specialized cold-chain shipping.
• Kit Optimization: Allows for the entire nucleic acid extraction kit to be stored and transported at ambient temperatures.
• Reliability: Guaranteed enzymatic efficiency even if temperature fluctuations occur during transit.

Conclusion

Tinzyme’s Recombinant RNase A Solution combines high-performance purity with industry-leading thermal stability. By eliminating DNase contamination and the need for refrigeration, we provide a robust, cost-effective solution for researchers and kit manufacturers worldwide.

Experience the stability of Tinzyme RNase A today.

1. Freeze/Thaw Cycle Test

This test evaluates the stability of dNTPs when subjected to repeated freezing and thawing, simulating frequent laboratory use.

2. Thermal Stability Test

This test measures the degradation of dNTPs when stored at room temperature (25℃) and elevated temperature (37℃) for 10 days.

3. Long-Term Stability Test

Data represents the purity maintained over a 5-year period under standard storage conditions.

In molecular biology experiments, we perform nucleic acid extraction and purification, which often involves various reagents.

Today, we will focus on Carrier RNA, also known as a nucleic acid coprecipitant.

1. Concept and Classification of Carrier RNA

Carrier RNA, also called Nucleic Acid Coprecipitant, refers to a mixture of RNA fragments ranging from 200 to 3000 nucleotides (nt). Common types include PolyA potassium salt, E. coli total RNA, yeast total RNA, and tRNA.

As an auxiliary substance for nucleic acid precipitation, Carrier RNA comes in several types:

1.1 Glycogen

Glycogen serves as an auxiliary precipitant for nucleic acids and generally performs better than tRNA or sonicated DNA. Since glycogen contains no DNase or RNase, it minimally affects subsequent PCR, RT-PCR, and restriction enzyme reactions. According to literature reports, ligation products precipitated with glycogen show virtually no interference with subsequent bacterial transformation. Glycogen at 0.001 mg/mL does not inhibit TdT (Terminal Deoxynucleotidyl Transferase), concentrations below 2 mg/mL almost never affect reverse transcriptase activity, and 0.02 mg/mL glycogen does not inhibit T4 RNA ligase activity. However, glycogen can interfere with DNA-protein interactions. Typically, 1 μL of 20 mg/mL glycogen solution is sufficient to precipitate picogram-level DNA or RNA from a 1 mL solution system.

1.2 Yeast tRNA Solution

When used at a final concentration of 10–20 µg/mL, yeast tRNA serves as an effective coprecipitant for trace nucleic acid recovery.
Since tRNA is a substrate for polynucleotide kinase and terminal transferase, tRNA cannot be used as a coprecipitant if the recovered DNA or RNA will be used for such reactions. If the recovered RNA is intended for RT-PCR, using tRNA may interfere with the specificity of the reaction.

1.3 Linear Acrylamide (LPA)

Linear Acrylamide, also known as Linear Polyacrylamide (LPA), is a coprecipitant that facilitates nucleic acid precipitation during purification processes. Essentially, acrylamide is a neutral carrier of non-biological origin, thus containing no potential nucleic acid or nuclease contamination. Additionally, it does not interfere with A260/A280 readings and does not inhibit polymerase or restriction endonuclease activities, making it compatible with subsequent molecular biology applications such as PCR and enzymatic digestion. Typically, 2-4 μL (10-20 μg) of 5 mg/mL solution is sufficient for precipitating DNA or RNA from 1 mL of solution.

2. Mechanism of Action

2.1 Reduction of Adsorption Losses: During nucleic acid extraction, electrostatic charges on centrifuge tube walls (typically polypropylene materials) can adsorb nucleic acids, leading to reduced extraction yields. The addition of Carrier RNA eliminates electrostatic effects, ensuring efficient binding of nucleic acids to purification columns and improving elution efficiency.

2.2 Decreased Nuclease Attack Probability: In extraction environments, RNases and other nucleases may degrade trace amounts of RNA. The presence of Carrier RNA reduces wall adsorption of target nucleic acids, thereby decreasing the probability of nuclease attack on the nucleic acids being extracted.

2.3 Enhanced Binding and Elution Efficiency: When RNA concentration is very low, minute amounts of RNA may be insufficient to form a precipitate. The addition of Carrier RNA provides adequate nucleic acid mass to facilitate precipitation formation.

3. Case Study

In plasma extraction, the primary challenge lies in the generally low content and small fragment size of cell-free DNA (cfDNA) in plasma samples.

We performed extraction from 200 μL of plasma, designing experiments with and without Carrier RNA to investigate its effect on the recovery efficiency of small fragment nucleic acid purification. Following the kit protocol, Groups 1 and 2 were supplemented with 5 μL of Carrier RNA, while Groups 3 and 4 received none. After extraction, fluorescent PCR amplification was performed to observe the results.
Analysis of the results shows that the CT values of samples 1 and 2 (with Carrier RNA) were approximately 1.5 cycles lower than sample 3 and about 3.5 cycles lower than sample 4. This indicates that in nucleic acid purification systems without Carrier RNA, the recovery efficiency of trace nucleic acids becomes unstable, ranging from 1/10 to 1/3 of the efficiency achieved in systems containing Carrier RNA.

4. When is Carrier RNA Essential?

We recommend adding Carrier RNA in the following three situations:

4.1 Extremely limited sample volume (e.g., swab wash fluid, trace serum)
4.2 Extremely low target nucleic acid concentration (e.g., early-stage viral infections, ctDNA/circulating tumor DNA)
4.3 When using ethanol precipitation methods (bead-based methods may not require it)

From: Molecular Biology Knowledge Base

As the Chinese New Year (Spring Festival) approaches, we would like to extend our warmest wishes to you and your family. Please be advised that our operations and shipping schedules will be adjusted during this festive period.

📅 Holiday Schedule

Our team will be taking a break to celebrate with their families on the following dates:

Office & Warehouse Holiday: [2026-02-15] – [2026-02-23]

Resume Normal Operations: [2026-02-24]

🚚 Shipping & Delivery Deadlines

Please note that logistics and courier services across China will also be taking a break. To ensure you receive your orders on time, please take note of the following:

Final Order Cut-off: All orders placed after [2026-02-11] will be processed but may not be shipped until we return.

Shipping Suspension: Local and international couriers will begin pausing services approximately 3-5 days before the official holiday starts.

Expect Delays: Any orders placed during the holiday period will be fulfilled chronologically starting from [2026-02-24]. Please expect a slight delay in delivery as we clear the holiday backlog.

❤️ Customer Support

While our shipping department is closed, our customer service team will have limited availability via email. We appreciate your patience and understanding if response times are longer than usual.

We suggest placing your orders as early as possible to avoid the holiday rush. Thank you for your continued support and for being a part of our community!

Wishing you a prosperous and Happy Year of the Horse!

Best Regards,

The Tinzyme Team

Product Code: PK01

We hereby declare that our Proteinase K (Product No. PK01) is manufactured under strict quality control standards and meets the following criteria:

• Animal-Free Composition: The product does not contain any ingredients derived from animal sources, nor does it contain any components contaminated by animal-derived substances.
• TSE/BSE Compliance: This product has not been exposed to animals affected by, or under quarantine for, Transmissible Spongiform Encephalopathy (TSE) or Bovine Spongiform Encephalopathy (BSE).
• Research Use Only: This product is designed exclusively for scientific research and laboratory experiments, specifically for protein degradation.

Disclaimer: This product is not intended for human or veterinary use, nor is it for use in food, cosmetics, or medicinal products. It is strictly for in vitro scientific research purposes.

PCM80
Universal FastStart Probe Mixture（UNG）

PCM31 Comparison of Amplification Effects of Different High Fidelity Mixes – Different Species, Different Models

① Brand A ② Brand B ③ TZ ④ PCM31

Newly designed model primers:
Human primers: H5k, H5k high GC, H10k
Mouse Primer: M5k, M5k, High GC.M10k

Newly designed model primers
Rice Primers: 05k, o5k – High GC, 010k
cDNA Primers: C-2.8k, C-4.5k

PCM80 Anti inhibition Performance Test: Application of Inhibitors (Ethanol, Blood, EGTA) Test Reagent for Anti inhibition Ability

There was no significant difference in CT values when adding 2mMEGTA as the final concentration. However, when adding 0.2% ASFV pig blood or 3% ethanol as the final concentration, the CT values advanced by 1-2 compared to 2xmix. Adding blood signals resulted in varying degrees of reduction (25% -28%).

PCM80 Digestive Ability Test

Application of human genome exploration and 2xmix (UNG) for one round of amplification (20cycles), followed by ten fold gradient dilution of one round of amplification products. The amplification products diluted by 103, 104, 105, 106, and 107 times were selected and amplified using no anti fouling system reaction solution and anti fouling system reaction solution, respectively. The CT differences at the same template concentration were compared.

Comparison of human genome template amplification:

Comparison of amplification of products containing dU in one round:

Conclusion: By amplifying 1ng/uL gDNA, PCM80 can be performed about 2 times earlier than UNG CT without anti fouling system; After amplifying the product containing dU in one round, PCM80 showed a significant CT lag effect compared to UNG without anti fouling system (CT difference greater than 10). When the product was diluted 106 times in one round, PCM80 did not amplify, indicating a direct relationship with the UNG system and strong digestive ability.