Manual

Product Number: RA01

Shipping and Storage

Sealed, dry.

Description

RNase A is an endonuclease that has been well studied and widely used. RNase A hydrolyzes RNA.

Application

1. Drugs: change host cell metabolism, inhibit virus synthesis,
2. Biochemical experiments: Catalyzed RNA degradation, which can specifically hydrolyze the RNA phosphate diester bond hybridized to THE DNA chain, so it can decompose the RNA chain in the RNA/DNA hybrid system.
3. Industrial production，decompose RNA and purify the product.

Specification

1. Source: Bovine (Porcine) pancreas.
2. Appearance: White or pale yellow lyophilized powder.
3. Solvent Transparentness: Not more opalescent than ref.suspensionⅡ.
4. Loss on drying: No more than 5.0%.
5. DNA: Not detectable.
6. Microbial limits: Pseudomonas aeruginosa: Negative; Salmonella: Negative; Staphylococcus aureus: Negative
7. Assay: No less than 50Kunitz units/mg
8. Protein (UV): No less than 65%

Order

Product Number: RA07

Shipping and Storage

Can be stored for 2 years at -20℃ to avoid repeated freezing and thawing.

Component

Description

RNase III can cleave longer double-stranded RNA (dsRNA) into a group of small interfering RNAs (siRNAs) of varying sizes, ranging from 18-25bp, making it more suitable for RNA interference experiments in mammalian cells. Using 1.5 units (1μL) of ShortCut RNase III can completely digest 1μg of dsRNA into siRNAs.

The E. coli RNase III produced by our company is a recombinant protein expressed through recombinant methods and purified through multiple steps.

Feature

1. Provide siRNA for RNA interference research
2. Target determination
3. Remove dsRNAs
4. Preparation of effective siRNA suitable for any gene target
5. Heterologous siRNA can ensure silencing of target genes
6. From DNA template to transfection only takes one day
7. Avoiding the problem of repeatedly exploring experimental conditions when using synthetic siRNA.

Unit definition

1 active unit refers to the amount of enzyme required to cleave 1μg dsRNA into siRNA in a 1 × E. coli RNase III reaction buffer system at 37 ℃ for 20 minutes.

Activity determination conditions

1 ×RNase III reaction buffer: 50mM Tris HCl, 50mM NaCl, 1mM DTT (pH 7.5 @ 25℃). Add 20mM MnCl2 (provided with the product) and incubate at 37℃.

Quality control

After strict quality control testing, the product is ensured to have the highest activity and purity.

Enzyme storage buffer

500mM NaCl，10mM Tris-HCl，0.5mM EDTA，1mM DTT，50% Glycerol，pH 8 @ 25°C

Product Number: GDH0601

Shipping and Storage

Suggest sealed storage, with a storage temperature not exceeding -20℃.

Description

This product is derived from microorganisms and catalyzes the dehydrogenation of glucose to produce gluconic acid, with advantages such as high specific activity and high yield. This product relies on FAD as a coenzyme, which has better stability and substrate specificity than other coenzymes such as NAD and PQQ.

Application

Glucose dehydrogenase (GDH) is a type of oxidoreductase that catalyzes the oxidation of glucose to form gluconic acid lipids. It has a wide range of applications in fields such as blood glucose detection, biofuel cells, implantable pacemakers, and industrial glucose content monitoring.

Features

1. Molecular weight: not less than 65kDa.
2. pI: 5.6
3. PH range: 5-9
4. Type: Freeze dried powder.
5. Temperature distribution: When the maximum activity value is 70℃, it is recommended to maintain a temperature range of 37-80℃.
6. Enzyme activity: >300U/mg freeze-dried powder.
7. Appearance: Yellow powder
8. Electrophoretic purity: >95% (SDS-PAGE)

Unit Definition

The enzyme activity unit (U) is the amount of enzyme that converts one micromole of 2,6-dichlorophenol indigo (DCPIP) per minute at pH 6.5 and 37℃. The enzyme activity values for each batch of products can be found in the corresponding quality inspection report.

Product Number: RA04

Storage

         Store at -20°C, with a minimum shelf life of 12 months.

Description

         Ribonuclease A, also known as RNase A, is an enzyme that digests RNA and is free of DNase. It is ready to use directly.

         It is used in various common molecular biology, cell biology, and related experiments.

Package

         1ml, 50ml.

Notes

         For your safety and health, please wear a lab coat and disposable gloves when handling.

Related products

•          CL30A, Plasmid Miniprep columns
•          PLC500, ME-tip500, Plasmid Maxi column (Resin)
•          LY03, lysozyme solution
•          PK02, Proteinase K solution

Product Number: RA02

Description

Our Ribonuclease A Solution is a non-specific ribonuclease derived from bovine pancreas that has been modified through protein engineering technology. It is obtained through yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is a ribonuclease that can hydrolyze the phosphodiester bond between the 5 ‘- ribose on a nucleoside and the phosphate group on the adjacent pyrimidine nucleoside 3’ – ribose. The resulting 2 ‘, 3’ – cyclic phosphate can be hydrolyzed into the corresponding 3 ‘- nucleoside phosphate. Therefore, single stranded RNA can be specifically degraded at the positions of C and U nucleotide residues. RNase A is very stable. RNase A exhibits high efficacy when acting on single stranded RNA.

This product is widely used to remove RNA contamination from DNA or protein samples.

This product is free from contamination by endonucleases and exonucleases, as well as protease contamination.

Quality Index

Note

For your safety and health, please wear lab clothes and protective gloves when using.

Product Number: ATA087

Shipping and Storage

-20°C.

Components

Description

Taq Antibody is a mouse monoclonal Antibody against Taq DNA Polymerase and applies to Hot Start PCR. When combined with the Taq DNA Polymerase, Taq Antibody inhibits DNA polymerase activity, which in turn inhibits non-specific annealing of primers and non-specific amplification induced by primer dimers at low temperatures. Taq Antibody denatures during the initial DNA denaturation step of the PCR reaction, when DNA polymerase activity is restored to achieve a heat-start effect. Therefore, there is no need for special inactivation of Taq antibody.

Features

1. At 37°C, >95% polymerase activity can be inhibited.
2. It can improve the specificity and sensitivity of PCR reaction, including complex human genomic DNA or cDNA template, low copy number DNA or RNA, multiplex PCR, etc.
3. PCR reaction rate is faster than common chemically modified polymerase.

Unit definition

After incubation at 25°C for 15 min, 1U Taq Antibody is defined to inhibit more than 97% of the 1U Taq DNA Polymerase activity at 37°C for 30 min.

Product Number: DI05

Shipping and Storage

 -20℃，Valid for 12 months.

Components

Description

DNase I is a non-specific nuclease cleavage enzyme, mostly derived from recombinant E. coli strains, containing MBP fusion clones of bovine pancreatic DNase I. DNase I can be used to degrade single or double stranded DNA, based on the principle that DNase I hydrolyzes phosphate diester bonds to produce single or oligonucleotides with 5 ‘- phosphate groups and 3’ – OH.

Both Mg²⁺ and Mn²⁺ can activate the activity of DNase I, and the concentration of Ca²⁺ directly affects the activity of the enzyme. When Mg²⁺ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn²⁺, double stranded DNA can be broken, resulting in DNA fragmentation.

DNase I solution (1mg/ml) is composed of DNase I, enzyme protection solution, preservatives, etc., with a concentration of 1mg/ml, used for single stranded DNA, double stranded DNA, chromatin, RNA: DNA hybrid strands. It is commonly used for the preparation of DNA free RNA, reverse transcription, and in vitro transcription experiments.

Unit definition

1 unit refers to the amount of enzyme required to completely degrade 1μg of pBR322 DNA in a 50μl reaction system at 37℃ for 10 minutes.

Recombinant grade, molecular biology grade, derived from microorganisms

Description

Unit Definition

The amount of enzyme required to generate 1 µmol of hydrogen peroxide (0.5 µmol of quinone imine dye) per minute is based on TOYOBO’s bioassay method.

Purity and Quality

Feature

Glucose Oxidase (Glucose Oxidase, GOD) is a yellow lyophilized powder obtained by fermenting and lyophilizing the Pichia pastoris gene of the Aspergillus niger glucose oxidase gene through genetic engineering, without animal-derived ingredients. In terms of function, GOD can convert β-D-glucose with high specificity, and the efficiency of other monosaccharides is very low. It has a fast deoxygenation speed, good thermal stability, and no precipitation or turbidity after the reaction is completed.

Application

In the application of the food industry, GOD can be used as a green and environmentally friendly additive in the food industry, which is non-toxic and has no side effects on the human body. For example, it can prevent beer from aging, maintain the original flavor of beer, and extend the shelf life; it can be used to improve the stability of red wine and grape juice; it can be used in packaged foods to deoxidize and extend the shelf life; it can also be used for milk, Preservation of fruit juices, vegetables, etc. Another important use of GOD is to determine the glucose content in a sample. Utilizing the principle of its specific oxidase, develop glucose oxidase analyzers or test paper products, which can quickly, accurately and easily measure the glucose content in various foods, and can also be used for the determination of human blood sugar. At present, glucose oxidase has been widely used in food industry, biomedicine industry and so on.

Note

Please use the specified buffer solution configuration, it is recommended to store below 4°C

Stability

The shelf life is at least 1 year when stored in a dry environment below 4°C after receipt

Inhibitor

p-chloromercuric benzoate (p-chloromercuribenzoate, pCMB) and heavy metal ions (Cu2+, Hg2+, Ag+, etc.)

Storage Buffer

20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl,50% Glycerin

Dilution Buffer

20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl

Notice and Disclaimer

For your safety and health, please wear a lab coat, protective gloves and goggles when using it.

This product is for research and development use only and is not intended for pharmaceutical, household or other use.

Donor:hydrogen-peroxidase oxidoreductase

DESCRIPTION

Appearance: Reddish-brown amorphous powder
Source: Horseradish
Enzyme Commission Number: EC 1.11.1.7
CAS Number: 9003-99-0
Storage temperature: -20℃
Activity: ≥250 Purpurogallin U/mg solid
Unit definition: One unit will form 1.0 milligram of purpurogallin from pyrogallol in 20 second at pH 6.0 at 20℃.

SPECIFICATION

Stability: Stable at -20℃ for at least one year
Molecular weight: ~40 kDa (SDS-PAGE)
Isoelectric point: 7.2
Optimum pH: 6.5
Optimum temperature: 45℃
pH Stability: 5.0~10.0(30℃, 16hr)
Thermal stability: < 50℃ (pH 6.0, 15min)
Inhibitors: NaN3

ORDER

E062013, Horseradish Peroxidase, CAS: 9003-99-0

DESCRIPTION

Appearance: Blue amorphous powder
Source: Plant
Enzyme Commission Number: EC 1.10.3.3
CAS Number: 9029-44-1
Storage temperature: -20℃
activity: ≥ 100U/mg solid
Unit definition: One unit causes the decrease of one micromole of ascorbic acid per min at pH 5.6 at 30℃.

SPECIFICATION

Molecular weight: 67kDa (SDS-PAGE)
Michaelis constant: 3.6×10^-4 M
Optimum pH: 6.0
Optimum temperature: 50℃
pH Stability: 5.5~9.5 (25℃, 20hr)
Thermal stability: < 50℃ (pH 7.0, 30min)
Inhibitors: Fe3+, Ni2+, BME, SDS, Proclin

ORDER

EAO030501, Ascorbate Oxidase, AAO, ASO, CAS: 9029-44-1, 1000KU