Cat: GOD0801

Recombinant grade, molecular biology grade, derived from microorganisms

Description

CAS No.	9001-37-0
Molecular mass	153kDa
pI	4.2
pH range	4-7
Storage temperature	Freeze-dried powder preparations can be transported at room temperature, and it is recommended to store in a dry environment at -20°C after receipt
Type	Freeze-dried powder
Temperature Distribution	40-50°C, the optimum temperature is 42°C
Enzyme activity	>50U/mg lyophilized powder

Unit Definition

The amount of enzyme required to generate 1 µmol of hydrogen peroxide (0.5 µmol of quinone imine dye) per minute is based on TOYOBO's bioassay method.

Purity and Quality

Exterior	Yellow powder
Electrophoretic purity	>90% (SDS-PAGE)
Enzyme activity	>50U/mg lyophilized powder

Feature

Glucose Oxidase (Glucose Oxidase, GOD) is a yellow lyophilized powder obtained by fermenting and lyophilizing the Pichia pastoris gene of the Aspergillus niger glucose oxidase gene through genetic engineering, without animal-derived ingredients. In terms of function, GOD can convert β-D-glucose with high specificity, and the efficiency of other monosaccharides is very low. It has a fast deoxygenation speed, good thermal stability, and no precipitation or turbidity after the reaction is completed.

Application

In the application of the food industry, GOD can be used as a green and environmentally friendly additive in the food industry, which is non-toxic and has no side effects on the human body. For example, it can prevent beer from aging, maintain the original flavor of beer, and extend the shelf life; it can be used to improve the stability of red wine and grape juice; it can be used in packaged foods to deoxidize and extend the shelf life; it can also be used for milk, Preservation of fruit juices, vegetables, etc. Another important use of GOD is to determine the glucose content in a sample. Utilizing the principle of its specific oxidase, develop glucose oxidase analyzers or test paper products, which can quickly, accurately and easily measure the glucose content in various foods, and can also be used for the determination of human blood sugar. At present, glucose oxidase has been widely used in food industry, biomedicine industry and so on.

Note

Please use the specified buffer solution configuration, it is recommended to store below 4°C

Stability

The shelf life is at least 1 year when stored in a dry environment below 4°C after receipt

Inhibitor

p-chloromercuric benzoate (p-chloromercuribenzoate, pCMB) and heavy metal ions (Cu2+, Hg2+, Ag+, etc.)

Storage Buffer

20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl,50% Glycerin

Dilution Buffer

20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl

Notice and Disclaimer

For your safety and health, please wear a lab coat, protective gloves and goggles when using it.

This product is for research and development use only and is not intended for pharmaceutical, household or other use.