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Biotin-11-dUTP 1mM Sodium solution

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Biotin-11-dUTP 1mM Sodium solution

Molecular Weight862.67 (free acid)
Molecular FormulaC28H45N6O17P3S (free acid)
Storage conditions-20℃
Purity≥95% (HPLC)
Package1mL, 10mL, 100mL, 1L


Biotin-11-dUTP is enzymatically incorporated into DNA, cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA, cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose, magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.

Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.

Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptase, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.


Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension


B11-D5331Biotin-11-dUTP 1mM Sodium solutionPurity≥95%

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