Product Number: BS03

Shipping and Storage

Store at -20°C.

Components

Description

WellStart III BST DNA polymerase is a hybrid enzyme containing WarmStafi Bst DNA polymerase and a thermostable reverse transcriptase. WarmStafi Bst III features modifications over Bst 3.0, eliminating nonspecific amplification during room-temperature reaction setup and eliminating the need for a separate activation step, thereby enhancing reaction specificity. The thermostable reverse transcriptase is a genetically engineered novel enzyme with significantly improved cDNA synthesis speed and thermal stability, capable of tolerating reaction temperatures up to 60°C, making it suitable for reverse transcription reactions with RNA templates possessing complex secondary structures. WellStart III BST DNA polymerase can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT-LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80 ℃ for 5 minutes to inactivate.

Product Number: BS07

Shipping and Storage

Transportation under 0°C and storage at -25~ -15°C.

Component

Description

Bst DNA polymerase V2 is derived from Bacillus stearothermophilus DNA Polymerase I, which has 5´→ 3´ DNA polymerase activity and strong chain replacement activity, but no 5´→3´ exonuclease activity. Bst DNA Polymerase V2 is ideally suitable for strand-displacement, isothermal amplification LAMP (Loop mediated isothermal amplification) and rapid sequencing. Well Start Bst DNA polymerase,large fragment is a hot-start version based on Bst DNA polymerase V2 obtained by reversible modification technology, which can inhibit DNA polymerase activity at room temperature, so the reaction system can be operated and formulated at room temperature to prevent non-specific amplification and improve reaction efficiency, and this version can be lyophilized. In addition, its activity is released at high temperatures, so there is no need for a separate activation step.

Application

1. LAMP isothermal amplification;
2. DNA strand single displacement reaction;
3. High GC gene sequencing;
4. DNA sequencing of nanogram level.

Unit Definition

One unit is defined as the amount of enzyme that incorporate 25nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Heat Inactivation

80℃, 20 min.

Product Number: BSM03W

Shipping and Storage

-20°C.

Components

Description

WellStart Bst 3.0 Enzyme Mix is a mixed enzyme that contains WellStart Bst DNA polymerase and high-temperature resistant reverse transcriptase.WellStart Bst 3.0 Enzyme Mix adds modifications on top of Bst 3.0, eliminating non-specific amplification generated during reaction establishment at room temperature and eliminating the need for a separate activation step, which can improve the specificity of the reaction.High temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering, with fast cDNA synthesis speed and significantly improved thermal stability. It can withstand reaction temperatures up to 60℃ and is suitable for reverse transcription reactions of RNA templates with complex secondary structures.WellStart Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80℃ for 5 minutes before inactivation.

Manual

Product Number: BS02

Shipping and Storage

-20°C.

Components

Description

WellStart II BST DNA polymerase is a recombinant enzyme expressed and purified by Escherichia coli. Its gene is derived from Bacillus stearothermophilus and has undergone partial point mutations based on the original sequence.This protein has stronger 5’→ 3′ DNA polymerase activity, chain displacement activity, reverse transcriptase activity, and no 5’→ 3′ exonuclease activity.Applied to DNA/RNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), etc.

Unit definition

The amount of enzyme required to add 10nmol of deoxynucleotides to acidic insoluble substances within 30 minutes at 65℃ is defined as 1 active unit (U).

Heat Inactivation

Incubate at 80°C for 5 minutes before inactivation.

Quality Control

After multiple column purification, the purity was detected by SDS-PAGE to be greater than 98%; No exogenous nuclease activity was detected.

Test

No activity at room temperature. Will be activated at 60°C.