Product Description

Vaccinia Capping enzyme is a recombinant protein from Vaccinia virus. Vaccinia Capping Enzyme can add 7-methylguanylate cap structure (Cap 0) to the 5′ end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, translation of mRNAs. Vaccinia Capping enzyme is composed of two subunits (D1R, D12L), and has three enzymatic activities (RNA triphosphatase, guanylyltransferase by the D1R subunit and guanine methyltransferase by the D12L subunit). All necessary for addition of a complete Cap 0 structure, m7Gppp5’N). In vitro, transcripts can be capped in the presence of reaction buffer, GTP, and the methyl donor (SAM).

Source

Recombinant E.coli

Concentration and Size

10U/μL

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10pmol of GTP into an 80 nt transcript in 1 hour at 37℃.

Storage Buffer

20mM Tris-HCl (pH 8.0), 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v/v) Triton X-100, 50%(v/v) glycerol.

Storage Conditions

-20 °C

Quality Assurance

Free of Endonuclease, Exonuclease, and RNase activities.

Physical Purity

Purity >95% by SDS-PAGE.

Order

This product is a GMP grade recombinant vaccinia virus mRNA cap 2′-O-methyltransferase. It can add a methyl group to the 2′-O of the first nucleotide of the cap structure (cap0) at the 5′ end of RNA to form an mRNA with cap1 structure. And it can only use RNA with 7-methylguanosine cap structure (m7gppppn) as the substrate. This product is a large-scale expression of recombinant vaccinia virus mRNA cap 2′-0-methyltransferase in E. coli, which conforms to GMP production and quality management specifications. All raw and auxiliary materials are traceable.

Specification

• Activity: ≥ 50 Ku / ml
• Purity: ≥ 95%
• Heavy metal residue: ≤ 10 ppm
• No common pathogens
• host protein residue: ≤ 50 ppm
• No residual RNase
• DNA residue in host: ≤ 100 pg / mg
• No residual endonuclease
• Endotoxin content: ≤ 10 EU / mg
• No nonspecific nuclease residue.

Order

This product is a GMP grade recombinant vaccinia capping system and related components. It can add 7-methylguanosine cap (m7gppp, cap0) to the 5′ end of RNA, which is closely related to the stability, transport and translation of mRNA.
The recombinant vaccinia virus capping enzyme in this product is expressed on a large scale in E. coli, which conforms to GMP production and quality management standards. All raw and auxiliary materials are traceable.

Specification:

• Host DNA residue: ≤ 100 pg / mg
• High purity: ≥ 95%
• No virus, mycoplasma and other common pathogens
• Heavy metal: ≤ 10ppm
• Internal toxicity control: ≤ 10eu / mg
• No RNase activity, endonuclease activity
• Host protein residue: ≤ 50 ppm
• No activity of exonuclease and protease

Order