Product Description

Luciferase is a general term for the enzymes that can produce biofluorescence in nature, the most representative of which is from the North American fluorescent worm (Photinus pyralis). The sequence of the firefly Luciferase mRNA (N1 Me Pseudo UTP) is from Photinus pyralis, and point mutation is carried out on the basis of the wild type sequence, significantly improving the stability of the protein and the suitable pH range. Once the product enters the cell, it will express Luciferase, Luciferase can catalyze the oxidation of the substrate D-luciferin to oxyluciferin. In the process of D-luciferin oxidation, bioluminescence will be generated at about 560nm wavelength and measured by a chemiluminescence meter or liquid scintillation meter. Firefly Luciferase is a commonly used bioluminescence reporter gene, which can be used as a control to study the translation efficiency of target genes in mammalian cells, Cell viability and in vivo imaging measurements. This product effectively reduces the autoimmunogenicity of mRNA in mammalian cells and enhances its stability by introducing N1-Me Pseudo UTP as a replacement for natural UTP. It also simulates mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃ with RNase Free Water as the storage buffer

Packing Size

Product Description

EGFP mRNA (N1 Me Pseudo UTP) encodes a green fluorescent protein, whose maximum excitation/emission light wavelengths are 488nm/509nm respectively. Transfected cells can express enhanced green fluorescence, which can be used as a control to study the transfection and expression of target genes in mammalian cells. This product can effectively reduce the autoimmunity of mRNA in mammalian cells by introducing N1 Me Pseudo UTP to replace natural UTP, Enhance the stability of mRNA while also simulating mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃

Product packaging

Product Number: M062003

Description

Specification

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N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.

Product Name

N1-Me-Pseudo-UTP, 100mM Solution

(N1-Me-Pseudouridine-5′-Triphosphate trisodium salt solution)

CAS No.

1428903-59-6

Molecular Formula

C₁₀H₁₄N₂Na₃O₁₅P₃

Molecular Weight

564.11

Molar absorptivity

8500 (λ=271nm)

Specification

Storage Recommendation

Please store in -20°C. Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

Quality Control

1. The concentration is verified by Uv-vis spectrophotometry.

2. This lot of N1-Me-Pseudo-UTP is free of DNase and RNase contamination.

3. The purity of this preparation is determined by HPLC.

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Description

• Product Name: Uridine 5′-triphosphate, sodium salt solution
• Molecular Formula: C₉H₁₂N₂Na₃O₁₅P₃
• CAS Number: 19817-92-6 (free acid)
• Molecular Weight: 550.09 (free acid)
• Appearance: Clear Colorless solution
• Concentration: 100mM±5%
• Purity(HPLC): ≥99%
• λmax: 262 nm±2 nm
• pH: 7.0±0.1

Applications

in vitro transcription. RNA amplification. siRNA synthesis. aRNA synthesis.

Quality Control Molecular Biology Grade

This lot of UTP has been performance tested in transcription reaction.
This preparation is free of DNase and RNase contamination as determined by incubation of the UTP with radioactive substrates.

Storage Recommendations

• Long term (Infrequent use; 1-2 times per month): -70°C
• Daily/Weekly use: -20°C
• Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
• These fluctuations can greatly alter product stability.
• The performance of this product is guaranteed for twelve months from the date of purchase.

rUTP solution is one component of 100mM rNTP set solution.
The rNTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP.

MOQ: 1ml of each
Delivery time: 2-3 working days after receive the payment.
Payment method: T/T or western Union or Money Gram full in advance.
OEM and Bulk both are available

Feature of the NTP

Greater than 99% purity confirmed by HPLC. Functionally tested in in vitro transcription. Highly stable – the neutral pH of nucleotide solutions ansures the stability during longterm storage.

Package & Delivery

Package Details:
1ml/ vial, 10ml/vial, 25ml 50ml 100ml 500ml, 1000ml and more

Delivery Time:
3 working days

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