Product Number:PCK54

Shipping and Storage

-30°C~ -15°C.Avoid repeated freeze-thaw cycles.

Component

Description

This product is a kit for one-step Real Time RT qPCR using RNA as a template using probe methods such as TaqMan and Molecular Beacon. When using this product for Real Time RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, without the need to add reagents or open the tube cap, which avoids contamination and improves experimental efficiency. This reagent introduces a dUTP/UG anti contamination system, which can rapidly degrade pollutants containing U at room temperature without affecting the efficiency and sensitivity of RT qPCR. This product is equipped with a freeze-drying protectant and can be used for the preparation of freeze-drying reagents.

Note

1. This product uses RNA as a template for one-step RT-PCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a dedicated area, using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with a 0.1% DEPC (diethyl carbonate) aqueous solution at 37 ℃ for 12 hours, and sterilized under high pressure for 30 minutes before use.
2. This product should avoid repeated freeze-thaw cycles and is recommended to be packaged and stored separately.
3. If there is precipitation or crystallization after melting the 5 × One Step Lyophilization Protector, please dissolve it in a 70 ℃ water bath without affecting its use.

Product Number: PCM355

Shipping and Storage

-20±5°C,try to avoid repeated freeze-thaw cycles.

Components

Description

Super Multiplex PCR Mix (UNG), Lyophilized is a premixed system suitable for various types of multiple PCR, with a concentration of 2.5×,Contains components such as DNA polymerase, UNG enzyme, PCR buffer, dNTPs, Mg²⁺, and enhancers.

Super Multiplex PCR Mix (UNG), a genetically modified recombinant enzyme containing DNA polymerase, has 5’→3′ DNA polymerase activity and no 5’→3′ exonuclease activity; DNA polymerase, modified by a new type of antibody, is an antibody modified hot start enzyme with high amplification efficiency. It also has excellent characteristics such as short activation time, strong amplification ability, and high sensitivity.This product introduces a dUTP/UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.

Super Multiplex PCR Mix (UNG) is suitable for anti pollution multiple PCR reactions, such as microsatellite analysis, genotyping, SNP detection, etc. This product can be paired with freeze-drying protectants for the preparation of freeze-drying reagents.

Notes

1. Before use, please gently mix the product upside down after it has completely melted, and centrifuge briefly before use.
2. Avoid repeated freeze-thaw of this product. Repeated freeze-thaw may cause a decrease in product performance, and it is recommended to store it separately.

Product Number: PCK49

Shipping and Storage

Stored at -30~-15℃, valid for 1 year. If frequent use is required, it can be packaged and stored at 2-8℃ to avoid repeated freeze-thaw as much as possible

Components

Description

This product is a specialized reagent kit for one-step RT-qPCR using probe methods (TaqMan, Molecular Beacon, etc.).When using this product for RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, and there is no need to add reagents or open the tube cap during the reaction process, which avoids pollution and improves experimental efficiency.This product has high detection sensitivity, strong fluorescence signal, and high signal-to-noise ratio, making it very suitable for the detection of trace amounts of RNA such as RNA viruses. The special buffer system contained in it can maximize the effectiveness of both reverse transcriptase and DNA polymerase, improving reaction efficiency.Using this product can obtain a wider linear range, more accurate quantification of the target gene, good repeatability, and high reliability. This kit introduces a dUTP-UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.UNG enzyme can rapidly degrade pollutants containing U at room temperature without affecting the formation of new dU based PCR products.

Notes

1. Before using the reagents in this kit, please gently mix them upside down to avoid foaming, and use them after brief centrifugation.
2. This product uses RNA as a template for one-step RT-qPCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a specialized area using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with 0.1% DEPC (diethyl pyrocarbonate) aqueous solution at 37℃ for 12 hours and sterilized under high pressure for 30 minutes before use.
3. The reagents in this kit should avoid repeated freezing and thawing; This product can be stored at -20℃ for long-term storage. If frequent use is required in the short term, it can be stored at 2-8℃.
4. This reagent kit must use specific primers, and the selection of primers can be based on specific experiments. The quality of primer design directly affects the results of RT-qPCR reaction. When designing primers, factors such as GC content, primer length, primer position, and the secondary structure of PCR products need to be considered. It is recommended to use professional primer design software for design.
5. It is recommended to use specific probes in this kit and use professional design software for design

Product Number:PCK27

Shipping and Storage

-30°C~ -15°C, transport ≤0°C.

Component

Description

Fast Probe One Step RT-qPCR, UNG Kit is designed specifically for quantitative PCR detection using RNA as a template, such as RNA viruses. Using gene-specific primers (GSPS), reverse transcription and qPCR reactions are performed in one tube without the need for additional tube opening/pipetting operations, greatly increasing assay throughput and reducing the risk of contamination. The dUTP/UNG anti-contamination system is introduced in this kit. Heat sensitive UNG can degrade U-containing pollutants rapidly at room temperature. Rapid inactivation of UNG by reverse transcription at 55°C did not affect the efficiency and sensitivity of qRT-PCR. Combined with optimized buffer system, antibody modified Taq enzyme and mutated M-MLV, the detection sensitivity of Fast Probe One Step RT-qPCR, UNG Kit can reach 0.1pg total RNA or <10 copies of RNA template and has higher thermal stability. 5×Fast Probe One Step RT-qPCR UNG Buffer contains optimized buffer system and dNTP/dUTP Mix, which is especially suitable for high specificity, low template concentration and multiple fast detection of fluorescent-labeled probes such as TaqMan.

Note

Before use, please gently mix the product upside down after it is completely melted, avoid foaming as far as possible, and use after a short centrifugation. Avoid freezing and thawing this product repeatedly. ROX dye is used to correct fluorescence signal errors generated between quantitative PCR holes. This product does not contain ROX dye.

Product Number: PCM15G

Shipping and Storage :

-20±5°C, if you need to use frequently, store at 2-8°C, try to avoid repeated freeze-thaw.

Components

Description

2×SNP Genotyping qPCR MIX, UNG is dedicated to probe-based SNP typing Real-time PCR premix systems at 2× concentrations, including Taq DNA Polymerase, PCR Buffer,dNTPs, Mg²⁺ and reinforcing agents and stabilizers are simple and convenient to operate, and this product is introduced dUTP/UNG antifouling Staining system, which greatly reduces false positives due to amplification products.Unique PCR buffer system on blood, saliva Complex templates such as liquid are highly tolerant and support direct expansion of oral swab fluid and blood with a final concentration of no more than 15%.There is no need for complicated extraction and preservation processes. Typing results are fast and accurate

Pre-experiment preparation and important notes

1. Please mix gently upside down before use, try to avoid foaming, and use after a short centrifugation.
2. Avoid repeated freeze-thawing of this product, repeated freeze-thawing may degrade the performance of the product. This product can be placed in for long-term storage Store at -20±5°C in the dark. If frequent use is required in the short term, it can be stored at 2-8 °C.

Product Number: PCM22

Shipping and Storage

-20±5˚C. Short term use can be stored in 2-8˚C. Try to avoid repeated freeze-thaw cycles as much as possible.

Components

Description

The 2×Animal Detection Probe Mixture, UNG (Mg²⁺ free) Lyophilized is a specialized freezeable intervention mixture that does not contain Mg²⁺ and is suitable for probe detection of DNA viruses, with a concentration of 2×,contains novel antibody modified Taq DNA polymerase, PCR buffer, dNTPs, as well as enhancers and stabilizers, making it convenient and fast to use. This product does not contain Mg²⁺, and the Mg²⁺content can be adjusted to meet different amplification needs.This product is compatible with single and multiple probe qPCR reaction systems, and can also be used for the preparation of freeze-drying detection reagents.

This product uses the dUTP-UNG anti pollution system, and dUTP is added during the preparation process of the PCR reaction system, resulting in the formation of amplification products containing dU bases.And this product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction. This effectively removes residual contamination of PCR products and greatly reduces false positives caused by amplification product contamination.The pre denaturation step of UNG enzyme in the PCR cycle can be inactivated, so it will not affect the formation of new dU based PCR products.

Product Number:PCM21

Shipping and Storage

Store at 4-30℃, and store at -20℃ until fully used after re dissolution, avoiding repeated freeze-thaw as much as possible..

Components

Description

Animal Detection Probe Mixture (UNG), Lyophilized is a specialized in situ full component freeze-drying reagent suitable for detecting DNA viruses using probe method. It includes novel antibody modified Taq DNA polymerase, PCR Buffer, dNTPs, Mg²⁺, as well as enhancers and stabilizers.Convenient and fast to use, simply add primer probes and extract the nucleic acid samples for machine amplification. This product is compatible with single and multiple probe qPCR reaction systems.

This product uses the dUTP-UNG anti pollution system, and dUTP is added during the preparation process of the PCR reaction system, resulting in the formation of amplification products containing dU bases.And this product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction.This effectively removes residual contamination of PCR products and greatly reduces false positives caused by amplification product contamination.The pre denaturation step of UNG enzyme in the PCR cycle can be inactivated, so it will not affect the formation of new dU based PCR products.

Note

1. This product can be stored at 4-30℃ for a long time, and at -20℃ for longer periods of storage. If it cannot be used up after re dissolution, it can be stored at -20℃ to avoid repeated freeze-thaw.
2. ROX dye is used to correct the fluorescence signal error generated between quantitative PCR wells, and this product does not contain ROX dye.

Product Number: PCM16

Storage Condition

-20 ± 5 ℃, for frequent use, 2×ASFV Identification qPCR Buffer UNG plus can be stored at 2-8 ℃ to avoid repeated freeze-thaw cycles.

Components

Description

ASFV Identification qPCR Mix UNG plus mainly includes 2×ASFV Identification qPCR Buffer UNG plus and 50 × Taq DNA Polymerase UNG plus (for ASFV) two parts, suitable for rapid fluorescence quantitative detection of African swine fever virus using probe method.

2×ASFV Identification qPCR Buffer UNG plus mainly includes optimized buffer systems, dNTPs, Mg2+, K+, enhancers, and stabilizers, etc,50× Taq DNA Polymerase UNG plus (for ASFV) includes Taq DNA Polymerase and Uracil-N-Glycosylase, among which Taq DNA Polymerase is a double antibody blocking hot start enzyme, which has 5 ′ -3 ′ DNA polymerase activity and 5 ′ -3 ′ Exonuclease activity, no 3 ′ -5 ′ Exonuclease activity, and is thermally stable. It still maintains 50% activity when kept at 94 ℃ for 1 hour. The enzyme extension speed reaches 2 kb/min, and the blocking rate of polymerase activity at 55 ℃ and below reaches more than 95%, It can effectively reduce non-specific amplification, ensure high specificity and precise quantitative analysis of PCR results, block the inactivation of antibodies during the denaturation stage, and do not require special inactivation treatment; Uracil-N-Glycosylase is inactive to RNA and can catalyze the release of free Uracil from single stranded and double stranded DNA containing Uracil, effectively eliminating system pollution. This product has strong stress resistance in amplification of low concentration blood samples, saliva samples, etc.

Note

1. Before use, please gently mix it upside down to avoid foaming, and use after briefly centrifuging.

2. Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored in a dark place at -20 ± 5 ℃ for long-term storage. If frequent use is required in the short term, 2×ASFV Identification qPCR Buffer UNG plus can be stored at 2-8 ℃.

Product Number: PCM34

Storage

-20°C, try to avoid repeated freezing and thawing.

Component

Description

2×Multiplex PCR MasterMix (UNG) is a PCR premix system composed of GoldStar Taq DNA Polymerase, Mg2+, dNTPs (including dUTP), UNG enzyme and PCR stabilizer. The use of this product does not require the optimization of complicated PCR reaction conditions, and multiple PCR reactions can be easily performed by simply exploring the conditions.

The GoldStar Taq DNA Polymerase contained in this product is a chemically modified hot-start enzyme, which can effectively reduce the non-specific amplification caused by primer mismatch in the early stage of PCR reaction. The unique buffer system enables all primers in the multiplex PCR reaction to be effectively extended without additional optimization. Also included in this MasterMix is the GC Enhancer, which facilitates efficient amplification of “difficult” templates (eg, those with high GC content). This product uses the dUTP-UNG anti-pollution system, which can effectively remove the residual pollution of PCR products and greatly reduce the false positives caused by the pollution of amplification products. The UNG enzyme can be inactivated by the pre-denaturation step in the PCR cycle, so it will not affect the formation of new dU-containing PCR products.

Multiplex PCR MasterMix (UNG) can effectively prevent residual contamination of PCR products, and is suitable for anti-pollution multiplex PCR reactions, such as microsatellite analysis, genotyping and SNP detection, etc.

Quality Control

After testing, there is no exogenous nuclease activity; PCR method detects no host residual DNA; storage at 2-8°C for 3 days, no significant change in amplification performance.

Product Number: PCM63, PCM63L, PCM63H

Storage Condition

-20°C. For frequent use, it can be stored at 2-8°C. Try to avoid repeated freezing and thawing.

Components

Description

2×HotStar Probe Mix UNG is a premix system dedicated to real-time fluorescent quantitative PCR with a probe method (TaqMan, Molecular Beacon, etc.), with a concentration of 2×, including HotStar Taq DNA polymerase, PCR Buffer, dNTPs (dTTP is all replaced by dUTP replaced), UNG enzyme and Mg2+, the operation is simple and convenient. It is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription, such as gene expression analysis, copy number analysis, SNP genotype analysis, etc. The dUTP-UNG anti-pollution system is used in this product, and dUTP is added in the preparation process of the PCR reaction system, so an amplification product containing dU bases is formed. This product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction. In this way, the residual contamination of the PCR product is effectively removed, and the false positive caused by the contamination of the amplification product is greatly reduced. The UNG enzyme can be inactivated by the pre-denaturation step in the PCR cycle, so it will not affect the formation of new dU-containing PCR products. The HotStar Taq DNA Polymerase contained in this product is a chemically modified, brand-new high-efficiency hot-start enzyme, which has no polymerase activity at room temperature, and effectively avoids non-specific binding of primers and templates or primer dimers at room temperature. To generate non-specific amplification, enzyme activation must be incubated at 95°C for 10 minutes. The unique combination of PCR buffer system and hot-start enzyme significantly improves the amplification efficiency of PCR, with stronger fluorescent signal and higher sensitivity, and can detect single-copy templates. Using this product can get a wider linear range and more accurate quantification of the target gene.

ROX dye is used to correct the fluorescence signal error between the wells of the quantitative PCR instrument, and is generally used in Real Time PCR amplification instruments of companies such as ABI and Stratagene. The excitation optics of different instruments are different, so the concentration of ROX dye must be matched with the corresponding real-time PCR instrument.

Instruments that do not require ROX calibration (PCM63):

Roche LightCycler 480, Roche LightCyler 96, Bio-radi iCyler iQ, iQ5, CFX96, etc.

Instruments requiring Low ROX calibration (PCM63L):

ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 etc.

Instruments requiring High ROX calibration (PCM63H):

ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One/Step One Plus etc.

Note

1. Before use, please mix it upside down gently, try to avoid foaming, and use it after short centrifugation.

2. Avoid repeated freezing and thawing of this product, as repeated freezing and thawing may reduce product performance. Long-term storage of this product can be stored at -20°C, away from light. If it needs to be used frequently in a short period of time, it can be stored at 2-8°C.