Product Number: TE035

Shipping and Storage

-20℃.

Components

Description

T5 Exonuclease is encoded by the D15 gene of T5 bacteriophage and can degrade DNA along the 5 ‘-3’ direction. It can be digested from both the 5 ‘end and the cleavage or gap of linear or circular double stranded DNA, but cannot degrade supercoiled DNA. The single stranded endonuclease activity of this enzyme can be inhibited by low concentrations of Mg^2+less than 1mM.

Application

1. Degradation of ssDNA and dsDNA;
2. Degradation of plasmid DNA with cleavage gaps;
3. When degrading single stranded and double stranded DNA, it can preserve the supercoiled plasmid DNA.

Features

1. Efficient and sensitive: Short degradation time, high degradation rate for single and double stranded DNA fragments, and sensitive response.
2. High quality: fully meets the experimental requirements for degrading single and double stranded DNA fragments.
3. Unique formula of 10 × Reaction Buffer: makes substrate degradation reaction more stable, sensitive, and efficient.