Product Number: PCM15G

Shipping and Storage :

-20±5°C, if you need to use frequently, store at 2-8°C, try to avoid repeated freeze-thaw.

Components

Description

2×SNP Genotyping qPCR MIX, UNG is dedicated to probe-based SNP typing Real-time PCR premix systems at 2× concentrations, including Taq DNA Polymerase, PCR Buffer,dNTPs, Mg²⁺ and reinforcing agents and stabilizers are simple and convenient to operate, and this product is introduced dUTP/UNG antifouling Staining system, which greatly reduces false positives due to amplification products.Unique PCR buffer system on blood, saliva Complex templates such as liquid are highly tolerant and support direct expansion of oral swab fluid and blood with a final concentration of no more than 15%.There is no need for complicated extraction and preservation processes. Typing results are fast and accurate

Pre-experiment preparation and important notes

1. Please mix gently upside down before use, try to avoid foaming, and use after a short centrifugation.
2. Avoid repeated freeze-thawing of this product, repeated freeze-thawing may degrade the performance of the product. This product can be placed in for long-term storage Store at -20±5°C in the dark. If frequent use is required in the short term, it can be stored at 2-8 °C.

Product Number: PCM15

Storage Condition

-20°C. For frequent use, it can be stored at 2-8°C. Try to avoid repeated freezing and thawing.

Product Component

Description

Multipurpose SNP Genotyping qPCR MIX is a real-time fluorescence quantitative 2×PCR premix system for probe-based SNP genotyping, including Taq DNA Polymerase, PCR Buffer, dNTPs, Mg²+, enhancers and stabilizers, and is easy and convenient to operate. The unique PCR buffer system has strong tolerance to complex templates such as blood and saliva. It can not only efficiently amplify the extracted DNA, but also support the direct amplification of buccal swab fluid and blood with a final concentration of no more than 15%. increase, without complicated extraction and storage process. Typing results are fast and accurate.

Pre-experiment preparation and precautions

1. Before use, please mix gently up and down, try to avoid foaming, and use after short centrifugation.

2. Avoid repeated freezing and thawing of this product, as repeated freezing and thawing may reduce the performance of the product. Long-term storage of this product can be stored at -20°C away from light. If it needs to be used frequently in a short period of time, it can be stored at 2-8°C.

Product Number: PCM16

Storage Condition

-20 ± 5 ℃, for frequent use, 2×ASFV Identification qPCR Buffer UNG plus can be stored at 2-8 ℃ to avoid repeated freeze-thaw cycles.

Components

Description

ASFV Identification qPCR Mix UNG plus mainly includes 2×ASFV Identification qPCR Buffer UNG plus and 50 × Taq DNA Polymerase UNG plus (for ASFV) two parts, suitable for rapid fluorescence quantitative detection of African swine fever virus using probe method.

2×ASFV Identification qPCR Buffer UNG plus mainly includes optimized buffer systems, dNTPs, Mg2+, K+, enhancers, and stabilizers, etc,50× Taq DNA Polymerase UNG plus (for ASFV) includes Taq DNA Polymerase and Uracil-N-Glycosylase, among which Taq DNA Polymerase is a double antibody blocking hot start enzyme, which has 5 ′ -3 ′ DNA polymerase activity and 5 ′ -3 ′ Exonuclease activity, no 3 ′ -5 ′ Exonuclease activity, and is thermally stable. It still maintains 50% activity when kept at 94 ℃ for 1 hour. The enzyme extension speed reaches 2 kb/min, and the blocking rate of polymerase activity at 55 ℃ and below reaches more than 95%, It can effectively reduce non-specific amplification, ensure high specificity and precise quantitative analysis of PCR results, block the inactivation of antibodies during the denaturation stage, and do not require special inactivation treatment; Uracil-N-Glycosylase is inactive to RNA and can catalyze the release of free Uracil from single stranded and double stranded DNA containing Uracil, effectively eliminating system pollution. This product has strong stress resistance in amplification of low concentration blood samples, saliva samples, etc.

Note

1. Before use, please gently mix it upside down to avoid foaming, and use after briefly centrifuging.

2. Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored in a dark place at -20 ± 5 ℃ for long-term storage. If frequent use is required in the short term, 2×ASFV Identification qPCR Buffer UNG plus can be stored at 2-8 ℃.

Description

Taq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus. The molecular weight of the product is approximately 94 kDa. EasyTaq DNA polymerase has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′ -5′ exonuclease activity. The extending speed is 1-2 kb/min. There is an “A” on 3′ end. The PCR product can be cloned in TA vector.

• CAS No. : 9012-90-2
• Other Names: nonstandard Taq DNA Polymerase
• Purity: 99.9%
• Classification: Specific Reagents
• nonstandard Taq DNA Polymerase: High concentration 100Unit

Package Details

• small package or bulk package both OK. Ship with blue ice and form box.
• storage buffer without glycerin.
• Offer OEM ODM and bulk service!
• high-sensitivity, high amplification Taq DNA polymerase. offer to all over the world, supply Bulk and OEM.

Characteristics

(1) high-sensitivity
(2) high amplification efficiency

Unit Definition

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Quality Control

• functional absence of double- and single-stranded endonuclease activity;
• Purity>90% homogeneous by SDS gel electrophoresis.
• Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

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Description

The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality.

Taq DNA polymerase: Has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′ – 5′ exonuclease activity. The extending speed is 1 kb/30 sec. There is an “A” on 3′ end. The PCR product can be cloned in TA vector.

Applications

• Hot-start PCR amplification
• Specific amplification of complex cDNA and genomic template
• Amplification from low copy number DNA template
• Real-Time PCR
• Multiple PCR
• Generation of PCR products for TA cloning, Gene microarray analysis and SNP test

Quality Control

• Functional absence of double and single-stranded endonuclease activity;
• Purity>99% test by SDS gel electrophoresis;
• Each lot of HotStar Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Concentration: 5 u/μl

Package: Bulk

Storage: Store at -20°C

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Product Number: PC09

Shipping and Storage

-20°C.

Components

Note: The 5×Golden Star Taq PCR Buffer of this product contains 8.5mM magnesium ions.

Description

Golden Star Taq DNA Polymerase is a chemically modified new and efficient Taq DNA Polymerase, which is completely blocked at room temperature, making the enzyme inactiveat low or normal temperature. Thus, non-specific amplification caused by non-specific binding of primers and templates or primer dimers can be effectively avoided at room temperature ,the activation of the enzyme must be incubated at 95°C for 10 minutes.The unique buffer system enables the enzyme to be widely used, and makes efficient amplification of templates with high GC content, complex secondary structure and low copy. Using this product for PCR amplification, the 3′ end of the PCR product with an “A” base, can be directly used for T/A cloning.This product has strong specificity and can be directly used for downstream cloning or chip hybridization experiments without the need for glue recovery to remove the heteroband after PCR amplification. It is mainly for conventional PCR, RT-PCR, real-time PCR, multiplex PCR, gene chip analysis and SNP detection, especially for PCR reaction with high specificity requirements.

Activity Definition

Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10nmol deoxynucleotides into acidic insoluble material was defined as 1 active unit(U) at 74°C for 30 min.

Quality Control

The purity of SDS-PAGE was greater than 99%. No exogenous nuclease activity was detected. No host residual DNA was detected by PCR. It can effectively amplify single copy genes in the human genome. There was no obvious activity change after one month storage at room temperature

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LA Taq DNA Polymerase three times higher than Taq DNA Polymerase

Long PCR Polymerase which synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone.

The fidelity of PCR is three times higher than with Taq DNA Polymerase.

The LA Taq is optimized for generation of very long amplicons: up to 40 kb with viral DNA and up to 15 kb with genomic DNA templates. The specially formulated LA Taq Buffer protects DNA from depurination and nicking during long thermal cycling.

The PCR products generated with the LA Taq DNA Polymerase are mostly 3′-dA tailed, which can be cloned in TA vector.

Features

• Long PCR products
• up to 40 kb with viral DNA as template
• up to 15 kb with genomic DNA as template
• Ideal for GC-rich templates up to 85% GC.
• Fidelity is three-times higher than with Taq DNA Polymerase.
• High yields. Incorporates modified nucleotides.

Quality Control

Functionally tested in generation of 40 kb amplicon with lambda DNA as template.

Concentration: 5 u/μl

Storage

Storage Buffer: The enzyme is supplied in: 20mM Tris-HCl (pH 8.0); 0.1mM EDTA; 1mM DTT; 100mM KCl; Stabilizers; 50% glycerol.

Storage: Store at -20°C.

Reaction

Reaction PCR Mixture Set Up and Recommended thermal cycling conditions: The flowing is only an example which take a 20KB human genomic DNA as the template, that only for a reference, It can be adjusted according the length and the sequence of the template and the primer.

94°C 3 min
94°C 30 sec
55°C 30 sec 30 cycles
72°C 1 min
72°C 5 min.

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Description

Taq Plus DNA Polymerase is a kind of High Fidelity Taq DNA Polymerase. mixture of Taq DNA Polymerase and proofreading DNA Polymerase, which allows for the amplification of long templates, up to 30kb, with high fidelity. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. PCR products, amplified up to 10kb in length with Taq Plus DNA Polymerase, generate a mixture of blunt ends and single base (A) 3′ overhang. The products can be used for direct T/A cloning, but its efficiency is not as high as PCR products amplified with Taq polymerase alone.

Taq Plus DNA Polymerase is a special formulation designed for amplify large fragment. The main component is Taq DNA Polymerase, and Pfu DNA Polymerase are added to enhance the efficiency of amplification reaction. Theoretically, Taq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1kb, and can amplify up to 20kb. Taq Plus also contains a proofreading activity that reduces the error rate of Taq Polymerase. Most of the amplified DNA fragments have a 3´A overhanging. However, a small percentage of the amplified DNA fragments are blunt-ended. Taq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.

Application

Long PCR (up to 20 kb), PCR cloning, RT-PCR etc.

Features

High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.

Higher yield: Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 20kb

Unit Definition

One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74°C

Quality Control

No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Concentration: 5u/µl

Storage

Storage Buffer: 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20.

Storage: Store at -20°C

10X Reaction Buffer (Mg²⁺ Plus)

500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton X-100, 100mM (NH4)₂SO₄, 15mM MgCl2, PCR enhancer

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Description

CSM Taq polymerases (Cold sensitive mutant Taq polymerases ) is a kind of HotStar Polymerase. Source from the mutant E. coli. CSM Taq polymerase unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq, Cold Taq is the cold sensitive mutant Taq. It retain the hot start ability throughout the whole amplification cycles.

Cold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites.

Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.

Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.

Cold sensitive mutant Taq polymerases are capable of extending through difficult regions, e. g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.

Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.

Applications

• Hot start PCR amplification
• Specific amplification of complex cDNA and genomic template, for amplification of difficult templates, such as GC-rich fragments and microsatellites
• Primer extension of SNP markers
• Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity
• Amplification from low copy number DNA template, high through-put Hot Start PCR with high specificity, sensitivity, and yield
• Routine diagnostic Hot Start PCR requiring high reproducibility.
• Real-Time PCR
• Multiple PCR
• Generation of PCR products for TA cloning

Quality Control

• Functional absence of double and single-stranded endonuclease activity;
• Purity>99% test by SDS gel electrophoresis;
• Each lot of CSM Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA ;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Concentration: 5 U/μl

Storage: Store at -20°C

Package: Bulk

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