Single-Stranded DNA Binding Protein, Thermostable SSBP, Increase the yield and specificitiy of PCR

Description

Thermo stable Single-Stranded DNA Binding Protein is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95°C for 60 minutes. Due to the extreme thermostability, thermaostable SSBP can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

Applications

RT-PCR & cDNA Synthesis

Product Source

An E. coli strain that carries the cloned ssb gene from a hyperthermophilic organism

Features

• Improve the processivity of DNA polymerase
• Stabilization and marking of ssDNA structure
• Increase the yield and specificitiy of PCR
• Increase the yield and processivity of RT during RT-PCR
• Improve DNA sequencing through regions with strong secondary structure

Transportation

on blue ice

Storage

at -20°C for 24 months

Order

Description

T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair. It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication. It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures. Recently, it has been shown to improve restriction enzyme digestion, improve the yield and efficiency of reverse transcription reactions during RT-PCR, enhance T4 DNA polymerase activity, as well as increase the yield of PCR products.

Applications

RT-PCR & cDNA Synthesis

Features

• Stabilization and marking of ssDNA structures
• Increase yield and processivity of RT during RT-PCR
• Increase yield and specificity of PCR products from soil samples

Transportation

on blue ice

Storage

at -20°C for 24 months

Order