Product Number: RA02

Description

Our Ribonuclease A Solution is a non-specific ribonuclease derived from bovine pancreas that has been modified through protein engineering technology. It is obtained through yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is a ribonuclease that can hydrolyze the phosphodiester bond between the 5 ‘- ribose on a nucleoside and the phosphate group on the adjacent pyrimidine nucleoside 3’ – ribose. The resulting 2 ‘, 3’ – cyclic phosphate can be hydrolyzed into the corresponding 3 ‘- nucleoside phosphate. Therefore, single stranded RNA can be specifically degraded at the positions of C and U nucleotide residues. RNase A is very stable. RNase A exhibits high efficacy when acting on single stranded RNA.

This product is widely used to remove RNA contamination from DNA or protein samples.

This product is free from contamination by endonucleases and exonucleases, as well as protease contamination.

Quality Index

Note

For your safety and health, please wear lab clothes and protective gloves when using.

Product Description

Luciferase is a general term for the enzymes that can produce biofluorescence in nature, the most representative of which is from the North American fluorescent worm (Photinus pyralis). The sequence of the firefly Luciferase mRNA (N1 Me Pseudo UTP) is from Photinus pyralis, and point mutation is carried out on the basis of the wild type sequence, significantly improving the stability of the protein and the suitable pH range. Once the product enters the cell, it will express Luciferase, Luciferase can catalyze the oxidation of the substrate D-luciferin to oxyluciferin. In the process of D-luciferin oxidation, bioluminescence will be generated at about 560nm wavelength and measured by a chemiluminescence meter or liquid scintillation meter. Firefly Luciferase is a commonly used bioluminescence reporter gene, which can be used as a control to study the translation efficiency of target genes in mammalian cells, Cell viability and in vivo imaging measurements. This product effectively reduces the autoimmunogenicity of mRNA in mammalian cells and enhances its stability by introducing N1-Me Pseudo UTP as a replacement for natural UTP. It also simulates mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃ with RNase Free Water as the storage buffer

Packing Size

Product Description

EGFP mRNA (N1 Me Pseudo UTP) encodes a green fluorescent protein, whose maximum excitation/emission light wavelengths are 488nm/509nm respectively. Transfected cells can express enhanced green fluorescence, which can be used as a control to study the transfection and expression of target genes in mammalian cells. This product can effectively reduce the autoimmunity of mRNA in mammalian cells by introducing N1 Me Pseudo UTP to replace natural UTP, Enhance the stability of mRNA while also simulating mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃

Product packaging

dNTP

Fluorescein-12-dUTP 1mM Sodium solution

Description

Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA, cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA, cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution. Fluorescein-12-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment and DNA Polymerase I.

Applications

Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

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dNTP

Biotin-11-dUTP 1mM Sodium solution

Description

Biotin-11-dUTP is enzymatically incorporated into DNA, cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA, cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose, magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.

Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5′-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.

Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptase, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.

Applications

Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension

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dNTP

Cy3-dUTP 1mM Sodium solution

Description

Cy3-dUTP can be used for direct enzymatic labeling of DNA, cDNA by PCR with Taq polymerase, Nick Translation with DNAse I, DNA Polymerase I, cDNA labeling and 3’-end labeling. Cy3-dUTP can replace TTP in reactions in which it serves as a substrate for T4 and Taq DNA polymerases, E. coli DNA polymerase (holoenzyme and Klenow fragment), reverse transcriptase (from AMV and MMLV) and terminal transferase. The synthesized dye-labeled DNA, cDNA probes can be used for the identification of specific sequences by in-situ hybridization, microarray or blotting techniques. Cy3-dUTP can also be used for multicolor fluorescence labeling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Cy3-dUTP, dTTP ratio for PCR and Nick Translation: 30-50% Cy3-dUTP, 50% dTTP.

Features

• High sensitivity.
• Low nonspecific binding.
• High photostability.
• High water solubility.
• pH insensitive.

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Product Number: M062003

Description

Specification

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N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.

Product Name

N1-Me-Pseudo-UTP, 100mM Solution

(N1-Me-Pseudouridine-5′-Triphosphate trisodium salt solution)

CAS No.

1428903-59-6

Molecular Formula

C₁₀H₁₄N₂Na₃O₁₅P₃

Molecular Weight

564.11

Molar absorptivity

8500 (λ=271nm)

Specification

Storage Recommendation

Please store in -20°C. Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

Quality Control

1. The concentration is verified by Uv-vis spectrophotometry.

2. This lot of N1-Me-Pseudo-UTP is free of DNase and RNase contamination.

3. The purity of this preparation is determined by HPLC.

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Description

dNTP Set consits of 100 mM aqueous solutions at pH 7.0 of each of dATP, dGTP, dCTP and dTTP, each in a separate vial.

Because the nucleotides are provided in separate solutions, dNTP sets offer maximum flexibility in preparation of reaction mixes for different applications.

Quality control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.
Greater than 99% purity of each component confirmed by HPLC.
Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities.

Applications

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling.
IVD use available!
For IVD customer we can offer bulk package!

Storage

Long Term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available

Delivery Time

2 working days

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