Product Number: SGS48

BDT 3.1 Sanger Sequencing Kit

Shipping and Storage

1. 5×Sequencing Buffer store at 2-8℃, BigDye3.1 store at -20℃.
2. Correctly stored, with a validity period of 12 months from receipt.

Components

Description

1. 5×Sequency Buffer and BigDye3.1 are both original products sold after packaging.
2. Sanger Sequency Mix is an optimized combination of 5×Sequency Buffer and BigDye3.1, containing a certain amount of auxiliary additives.
3. In theory, 1mL BigDye3.1 needs to be paired with 2-3mL 5×Sequency Buffer.
4. Purpose and scope of application: First generation sequencing reaction.

Note

1. The actual cost of the unit is relatively high, be careful when adding samples to reduce sample loss.
2. Before each experiment, perform instantaneous centrifugation to further reduce the loss of the tube wall.

Product Number: SGS3101, SGS3102

Components

This kit includes Sanger Dye ready reaction mix and 5x Sequencing buffer.

1. Avoid excess (that is, no more than 5-10) freeze-thaw cycles.
2. Before each use of the kit, allow the frozen stocks to thaw at room temperature (do not heat).
3. Mix each stock thoroughly and then centrifuge briefly to collect all the liquid at the bottom of each tube.
4. Whenever possible, keep thawed materials on ice during use. Do not leave reagents at room temperature for extended periods.

Description

Sanger sequencing, also known as chain termination sequencing, is a method of DNA sequencing developed by Frederick Sanger and colleagues in 1977. Sanger sequencing is the gold standard for both first-generation and next-generation sequencing. It is currently the international standard for all gene testing, including fluorescence quantitative PCR TaqMan probe method, ordinary PCR method, chip method, second-generation sequencing method, mass spectrometry method, and other methods.

The Sanger sequencing kit provides the required reagent components for the sequencing reaction in a ready reaction, pre-mixed format. You need only provide your template and the template-specific primer.

These reagents are suitable for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, on polymerase chain reaction (PCR) fragments, and on large templates (for example, BAC clones).

Keywords

Sanger Sequencing kit, big dye kit.