Product Number: RT07

Shipping and Storage

Store at -30 ~ -15 ℃ and transport on dry ice.

Components

Description

Super FiV M-MLV Reverse Transcriptase (RNase H-) is a reverse transcriptase that recombines and expresses mutated M-MLV genes in E. coli engineering bacteria. This enzyme can catalyze complementary DNA polymerization reactions using RNA or DNA: RNA hybrid chains as templates. The mutated Super FiV M-MLV Reverse Transcriptase (RNase H-) RNase H activity is missing, reducing RNA degradation in reverse transcription reactions and making it easier to obtain full-length cDNA. Super FiV M-MLV Reverse Transcriptase (RNase H-) exhibits excellent reverse transcription activity at 55 ℃ (the enzyme can be used for reverse transcription reaction at up to 60 ℃). For complex RNA structures, increasing the reverse transcription reaction temperature can significantly improve the yield of cDNA. In addition, the mutated Super FiV M-MLV Reverse Transcriptase (RNase H-) has better stability and can synthesize up to 15kb of cDNA. Suitable for the synthesis of first stranded cDNA, RT PCR, RT qPCR, and construction of full-length cDNA libraries.

Unit definition

Using Poly (A) as a template and oligo (dT) as a primer, the enzyme required to catalyze the addition of 1 nmol of dTTP within 10 minutes at 37 ℃ is defined as one active unit (U).

Quality control

200 U of this enzyme reacted with 1µg of 16S, 23S rRNA at 37 ℃ for 1 hour, and the electrophoresis band of the RNA remained unchanged.