Product Number: RT05

Shipping and Storage :

-20°C

Components

Description

HiFi II M-MLV(H-) is a reverse transcription enzyme in which the mutant M-MLV gene is recombined and expressed by Escherichia coli engineering bacteria. The enzyme can catalyze the polymerization of complementary DNA using RNA or DNA: RNA hybrid chain as template. The mutation of HiFi II M-MLV reverse transcriptase RNase H activity is lost, reducing the degradation of RNA in reverse transcription and facilitating the acquisition of full-length cDNA. HiFi II M-MLV reverse transcriptase can synthesize the first strand cDNA at 55°C, providing higher specificity and stability, and can synthesize up to 12 KB cDNA with high cDNA yield. It is suitable for synthesis of first strand cDNA, RT-PCR, RT-QPCR and construction of full-length cDNA library.

Activity Definition

Using Poly (A) as template and Oligo (dT) as primer, the amount of enzyme required for catalytic incorporation of 1 nmol dTTP within 10 minutes was defined as an activity unit (U) at 37°C.

Quality Control

The electrophoretic bands of RNA did not change after the reaction of 200 U of this enzyme with 1 μg of 16 S and 23 S rRNA at 37°C for 1 hour.