RNase R

Product Number: RR01

Shipping and Storage

Store at -20°C to avoid repeated freeze-thaw cycles.

Component

Description

Ribonuclease R (RNase R) originates from the E. coli RNR superfamily and is a magnesium dependent 3 ‘-5’ exonuclease that can gradually cleave RNA into dinucleotides and trinucleotides from the 3 ‘-5’ direction. RNase R can digest almost all linear RNA, but it is not easy to digest circular RNA, lasso structure RNA, and short double stranded RNA molecules with less than 7 nucleotides protruding from the 3 ‘end.

RNase R is commonly used in gene expression and variable splicing studies, and can digest linear RNA to enrich circular RNA or lasso structure RNA.

Application

1. Gene expression research;
2. Variable shear research;
3. Enriching circRNA from biological samples;
4. Identify intronic lasso structure RNA;
5. Identify exon circRNA.

Storage Buffer

50mM Tris-HCl, 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100 and 50% Glycerol (pH 7.5 at 25℃).

Definition of active units

The enzyme required to convert 1µg poly (A) into acid soluble nucleotides in 10 minutes under standard reaction conditions at 37℃ is defined as 1U.