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	<title>RE0581 &#8211; Tinzyme</title>
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		<title>BsmBI</title>
		<link>https://www.tinzyme.com/endonuclease/bsmbi/</link>
		
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		<pubDate>Tue, 27 May 2025 06:33:18 +0000</pubDate>
				<category><![CDATA[Endonuclease]]></category>
		<category><![CDATA[RE0581]]></category>
		<guid isPermaLink="false">https://www.tinzyme.com/?p=6520</guid>

					<description><![CDATA[RE0581, BsmBI
BsmBI belongs to the Type IIs restri...]]></description>
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<p><a href="https://www.tinzyme.com/man/RE0581.pdf" target="_blank" rel="noreferrer noopener">Manual</a></p>



<p><strong>Product Number: RE0581</strong></p>



<p><strong>Shipping and Storage</strong></p>



<p>-20℃.</p>



<p><strong>Components</strong><strong></strong></p>



<figure class="wp-block-table"><table class="has-fixed-layout"><tbody><tr><td>Component</td><td>Specifications V</td><td>Specifications S</td></tr><tr><td>BsmBI(10U/μL)</td><td>20μL</td><td>100μL</td></tr><tr><td>10×Cut Buﬀer C</td><td>1mL</td><td>1mL</td></tr></tbody></table></figure>



<p><strong>Description</strong></p>



<p>BsmBI belongs to the Type IIs restriction enzyme, which can recognize non palindromic sequences and perform cleavage outside of the recognized sequence. It is commonly used for Golden Gate assembly. The optimized reaction buffer maximizes the functionality of BsmBI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.</p>



<p><strong>Suggested reaction conditions</strong></p>



<ol class="wp-block-list">
<li>1 x HN buffer solution;</li>



<li>Incubate at 55℃;</li>



<li>Prepare the reaction system according to the &#8220;DNA enzyme digestion process&#8221;.</li>
</ol>



<p><strong>Inactivation conditions</strong></p>



<p>Incubate at 80℃ for 20 minutes.</p>



<p></p>
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