Product Number: P1331

Storage

Poly A RNA carrier is temperature stable, can ship at room temperature. Store at -20℃, Keep away from sunlight, sealed in dry place.

Description

Poly A is a synthetic nucleic acid sequence, which increase the yield of RNA or DNA during extraction process. Ethanol precipitation is commonly used to recover DNA and RNA from liquid samples. However, ethanol precipitation cannot completely recover the nucleic acid from the sample, at least 30% of the nucleic acid will lost. If the concentration of nucleic acid in the liquid sample is very low or DNA < 200bp, only 50% DNA and RNA can be recovered by ethanol precipitation. Poly A carrier is a type of synthetic polymer. Adding Poly A carrier in ethanol precipitation can significantly improve the yield of nucleic acid precipitation, and the recovery rate of trace DNA can reach 95-98%. At the same time, short primer (< 22bp) fragments and dNTP can be selectively removed for precipitation and recovery of labeled probes, and untagged dNTP can be removed. Compared to biologically derived nucleic acid precipitation aids such as glycogen and tRNA, Poly A carrier itself is free of nucleic acid pollution, DNase and RNase activity, and does not affect digestion, connection, transcription, PCR, transformation and transfection, nor does it affect DNA electrophoresis and DNA protein interaction. Poly A carrier has become the most commonly used nucleic acid precipitant.

Package

1g, 5g, or bulk.

Working concentration

A concentration of 0.5 mg/ml is recommended.

Features

• 1. Significantly improve the yield of DNA or RNA precipitation.
• 2. The recovery of trace DNA and RNA (20pg) was 95-98%.
• 3. It does not affect the enzyme cutting, linking, transcription, PCR and other reactions.
• 4. It does not affect the interaction between electrophoresis and DNA protein.

Note

Poly A carrier will increase the optical density value of RNA or DNA. Thus, when measuring the optical density value, to eliminate the influence of Poly A carrier, a blank control sample should be made according to the same experimental process (use the same reagent and Poly A carrier, but do not contain RNA or DNA samples, and dissolve the final Poly A carrier precipitation in the same solution as the sample). The optical density values of sample and blank control were measured at 260 and 280 nm. The actual optical density of the sample can be obtained by subtracting the optical density of the blank control from that of the sample. If the quantification does not need to be very accurate, it can also be estimated.

Product Number: PCM355

Shipping and Storage

-20±5°C,try to avoid repeated freeze-thaw cycles.

Components

Description

Super Multiplex PCR Mix (UNG), Lyophilized is a premixed system suitable for various types of multiple PCR, with a concentration of 2.5×,Contains components such as DNA polymerase, UNG enzyme, PCR buffer, dNTPs, Mg²⁺, and enhancers.

Super Multiplex PCR Mix (UNG), a genetically modified recombinant enzyme containing DNA polymerase, has 5’→3′ DNA polymerase activity and no 5’→3′ exonuclease activity; DNA polymerase, modified by a new type of antibody, is an antibody modified hot start enzyme with high amplification efficiency. It also has excellent characteristics such as short activation time, strong amplification ability, and high sensitivity.This product introduces a dUTP/UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.

Super Multiplex PCR Mix (UNG) is suitable for anti pollution multiple PCR reactions, such as microsatellite analysis, genotyping, SNP detection, etc. This product can be paired with freeze-drying protectants for the preparation of freeze-drying reagents.

Notes

1. Before use, please gently mix the product upside down after it has completely melted, and centrifuge briefly before use.
2. Avoid repeated freeze-thaw of this product. Repeated freeze-thaw may cause a decrease in product performance, and it is recommended to store it separately.

Product Number: PK01                           Lyophilized powder     Molecular bio grade

Shipping and Storage

Shipping under room temperature and Store at 4 to -20°C recommended.

Description

Proteinase K is a broad-spectrum serine protease originally isolated from fungus Engyodontium album. Proteinase K is commonly used in molecular biological and biopharmaceutical applications to remove protein contamination from preparations of highly native undamaged nucleic acid because it rapidly and effectively inactive nuclease that might degrade the DNA or RNA even in the presence of denaturing reagents. MEBEP recombinant proteinase K is a mutant to the native protease that result in improved specific activity, higher yield and wider range of pH/temperature with optimal activity. The large scale recombinant preparation has advantages in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating DNA and RNA templates. Recombinant proteinase K is widely used for general digestion of proteins and Chemo-enzymatic peptide synthesis in molecular biology, molecular diagnostic and biopharmaceutical applications. Proteinase K is one of important enzymes in various industries like leather, cosmetics, food and feed etc.

Preparation

1. The Molecular Biology Grade lyophilized powder is NOT sterile.
2. Stock solution can be prepared as 40-80 mg/ml in dilution buffer [20mM Tris-HCl (pH 7.4) , 1 mM CaCl₂] or [20mM Tris-HCl (pH 7.4) , 1 mM CaCl₂, 2% Glycerol], sterilized using a 0.22 μm filter and supplied at final concentration of 20-40mg/ml in 50% sterilized Glycerol. Store in aliquots at wide temperature range from 24°C to -80°C.
3. PES or PVDF membranes with low protein binding are recommended in sterile filtration.

Quality Control

1. DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37°C.
2. RNase Activity: none detectable of ribonuclease activity after 16 hours incubation at 25°C.
3. Protein Purity: > 95% (Native-PAGE and SDS-PAGE assay).

Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.

RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.

A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.

• CAS No.: 9001-99-4
• E.C.: 3.1.27.5
• Source: Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
• Activity: ≥40 Kunitz units/mg Protein
• Molecular weight: 14.3 kDa
• Form: Lyophilized Powder
• Grade: Molecular biology level
• Purity: ≥ 90%; (SDS-PAGE)
• Endotoxin: None detected (Tachypleus Amebocyte Lysate)
• Foreign activity: Endonuclease and exonuclease, none detected
• NICKase and DNase, none detected
• Optimal pH: 7.6 (activity range of 6-10)
• Optimal temperature: 60°C (activity range of 15-70°C)
• Shelf life: 3 years (-20°C)

Transportation and Storage

Storage temperature: -20°C.

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Description:

HotStar Probe One Step RT-qPCR lyophilized mix is designed for one-step qPCR based on probes (TaqMan, Molecular Beacon etc.). The reverse transcription and quantitative PCR are carried out in the one reaction system, no need to add other reagents in the whole reaction process. HotStar Probe One Step RT-qPCR mix is highly sensitive, strong fluorescent signal and highly signal-to-noise ratio. suitable for IVD use or research use.

This product can be used for rapid testing and R&D of RNA molecular products, and also suitable for RNA multiple products. After adding primer probe and water re dissolving, it is a full component detection reagent.

Quality Control:

No exogenous nuclease activity, no host DNA detected by PCR, genomic single copy DNA can be amplified effectively.

Shipping And Storage:

Ship at room temperature, no need dry ice or ice bag.

Bovine pancreatic deoxyribonuclease I (DNase I) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3′ position and phosphate group at the 5′ position.

The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca²⁺.

The Deoxyribonuclease I (DNase I) has been chromatographically purified to remove trace contaminating proteases. DNase I may be used in clinic to thin and liquefy mucus in the lungs.

Specification

Activity ≥ 2,000 Kunitz units/mg powder

High quality DNase I
High purity DNase I offer OEM and bulk package.
High quality Deoxyribonuclease I

DNase and RNase manufacturer offer OEM and bulk!

Storage

Store in an airtight container, at a temperature of 2°C to 8°C

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The Lyophilized MMLV Reverse Transcriptase uses advanced lyophilization technology made to lyophilized powder format. The lyophilized MMLV can ship and keep at room temperature, it is very stable under RT without any activity lost. No need ship with dry ice, ice bag and other cold chain. Save much shipping cost, also no need to worry about the shipment delay cause the enzyme change to bad.

The Lyophilized MMLV Reverse Transcriptase is also a thermal stable reverse transcriptase. The thermal MMLV reverse transcriptase is recombined and expressed by mutant M-MLV gene using E. coli engineered bacteria. Thermal stable MMLV reverse transcriptase can be used in cDNA synthesis with RNA or RNA: DNA hybrids as templates. Loss of RNase H activity of Thermal stable MMLV reverse transcriptase reduces the degradation of RNA templates and makes it easier to obtain full-length cDNA. Thermal stable MMLV reverse transcriptase can synthesize the first strand of cDNA at 55°C, with higher specificity and stability, and can synthesize up to 12kb cDNA, with high cDNA yield. Thermal stable MMLV reverse transcriptase is suitable for Synthesis of first-strand cDNA, RT-PCR, RT-qPCR and the construction of full-length cDNA library, etc.

Feature

• Lyophilized format, convenience for transportation
• Thermal stable
• High Specificity
• High Sensitivity

Component

Storage

Store at room temperature.

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Description

Storage Conditions

Store at 4 to -20ºC recommended.
Mutated Proteinase K powder is stable at room temperature in 3 years.
Expiration: Three years if kept below 4ºC.

Dilution Buffer 1 (short time storage): 20 mM Tris-Cl (pH 7.4), 1 mM CaCl2, 15% glycerol, or d3H2O.
Dilution Buffer 2 (long term storage): 20 mM Tris-Cl (pH 7.4), 1 mM CaCl2, 50% glycerol, 0.02-0.1% Sodium azide.

QC Assays

• Activity Assay and Unit Definitions: One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37º C, pH7.5.
• DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37ºC.
• RNase Activity: none detectable of ribonuclease activity after 16 hours incubation at 25 º C
• Protein Purity: over 99% (Native-PAGE and SDS-PAGE assay)

Applications

The application of this Mutated Proteinase K is similar with wild type Proteinase K. But the mutated one has higher specific activity and more stable at RT. It is a broad substrate non-specific serine Proteinase. It is active over the pH range 4-11. It was used on any situation to digest native and denatured proteins. For instance, it is used for isolating mRNA or genomic DNA from different tissues and modifying glycoprotein for structure studies. Mutated Proteinase K is active with SDS, urea and EDTA and active between the temperature15-75ºC

Other Names

• Proteinase K
• endopeptidase K
• Tritirachium alkaline proteinase
• Tritirachium album proteinase K
• Tritirachium album serine proteinase

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E Taq Master Mix Lyophilized Powder

Description

E Taq Master Mix (Lyophilized Powder) uses advanced lyophilization technology made to lyophilized powder format. The lyophilized E Master mix and the 2x E Master buffer for LP when keep at room temperature is very stable, without any activity lost. The 2x E Taq Master Mix contain E DNA Polymerase, Mg²⁺, dNTPs and special stabilizer and PCR enhancer, concentration is 2x E Taq DNA Polymerase has the Excellent performance of high Amplification Efficiency and low mismatch rate. The original Master Mix formula makes the reaction system very stable, more than 98% PCR amplification can be successful at one time. At the same time, the complex template can be amplified effectively and minimize human error and pollution. This product does not contain dyes. After the completion of the PCR procedure, a suitable amount of sample loading buffer is needed according to the need for electrophoresis operation. Most of the amplified products have a “A” base at the 3′ end, so they can be directly used in T/A cloning.

Packing size

1ml or 5ml

Components

1vial E Taq Master Mix LP with 1vial of dilution buffer

Quality Control

No exogenous nuclease activity, no host DNA detected by PCR, various genomic single copy DNA can be amplified effectively.

Protocol

Pre preparation Add 2x E Taq Master buffer for LP to E Taq Master LP, Vortex oscillation until complete dissolution.
After it completely dissolved, transferre the solution from cillin bottle to a 2x Es Taq Master buffer for LP tube, then make a mark on it.
The mixed solution needs to be stored at -20°C for a long time storage, if frequency use, it can be stored at 2-8°C.
The following example is applied to conventional PCR reaction system and reaction conditions and it can be modified and optimized according to the template, primer structure and target fragment size.
Template DNA Human genomic DNA, size 1KB

Shipping And Storage

E Taq Master LP and 2x E Taq Master buffer for LP can store at room temperature.
When E Taq Master LP is mixed with 2 x E Taq Master buffer for LP, it needs to be stored at -20°C
If frequently use, Store at 2-8°C for short-term (1 week), and avoid freeze thawing

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