Product Number: PH29

Shipping and Storage

-20°C

Components

Description

Phi29 DNA Polymerase is a DNA Polymerase cloned from Bacillus subtilis phage Phi29 and expressed by Escherichia coli using gene recombination technology. This product has high-efficiency DNA continuous synthesis ability and strand displacement ability, as well as 3’→5′ exonuclease proofreading function, with high fidelity. This product can be used in replication reactions that require strong displacement and continuous synthesis, and high-fidelity replication under mid-temperature conditions, such as plasmid replication, whole genome amplification, etc.

Unit definition

The amount of enzyme required for incorporation of 0.5 pmol of deoxynucleotide into the acid- insoluble precipitate at 30°C for 10 min was defined as 1 activity unit (U).

Heat Inactivation

Inactivation after incubation at 65°C for 10 min.

Quality Control

After several column purification, SDS-PAGE detection of its purityis greater than 95%. No endonucl ease activity was detected and no host DNA was left.

Note

The enzyme buffer contains the reducing agent DTT to ensure maxi mum enzyme activity. If the buffer is not fresh or has been repeatedly freeze-thaw, 4mM of DTT shoul d be added before use.