Fast NGS Library Kit for Illumina

tinzyme — Mon, 13 Jan 2025 00:58:44 +0000

Product Number: PCK85

Storage

Store at -20 ℃ and transport on dry ice.

Components

Component	PCK85	PCK85
End Prep Enzyme Mix	48 μl	192 μl
10×End Repair Reaction Buffer	200 μl	800 μl
T4 DNA Ligase	48 μl	192 μl
T4 DNA Ligase Buffer	400 μl	2×800 μl
2×HiFidelity PCR Mix	600 μl	2×1.2 ml

Description

This kit provides the enzyme premix system and reaction buffer required for DNA library construction, including all components except connectors and PCR primers, for the construction of Illumina second-generation sequencing platform DNA library. Compared with general library building methods, this kit is simple and convenient to operate, greatly shortening the library construction time. In addition, the kit uses high-fidelity DNA polymerase for library enrichment, with no preference for PCR amplification, expanding the coverage area of the sequence, and can efficiently prepare a DNA library for the Illumina second-generation sequencing platform. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability of library construction to the greatest extent possible.

Features

End leveling, phosphorylation, and addition of A are completed in one step.
No need for purification, add connectors directly.
Ultra fidelity amplification minimizes amplification preference.
Supports multiple samples, and the resulting library can be used for sequencing platforms such as Illumina GAIIx, HiSacnSQ, HiSeq 2500/2000/1000, and MiSeq sequencing.

Schematic diagram of DNA database construction process

PCK85, Fast NGS Library Kit for Illumina, Schematic diagram of DNA database construction process

PCK85 – Tinzyme

Fast NGS Library Kit for Illumina