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	<title>PCK244 &#8211; Tinzyme</title>
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		<title>Single Cell WGA Kit</title>
		<link>https://www.tinzyme.com/ngs/single-cell-wga-kit/</link>
		
		<dc:creator><![CDATA[tinzyme]]></dc:creator>
		<pubDate>Mon, 13 Jan 2025 03:35:36 +0000</pubDate>
				<category><![CDATA[NGS]]></category>
		<category><![CDATA[PCK244]]></category>
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					<description><![CDATA[PCK244, Single Cell WGA Kit
The single-cell whole ...]]></description>
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<p><a href="https://www.tinzyme.com/man/PCK244.pdf" data-type="link" data-id="https://www.tinzyme.com/man/PCK244.pdf" target="_blank" rel="noreferrer noopener">Manual</a></p>



<p><strong>Product Number: PCK244</strong></p>



<p><strong>Shipping and Storage</strong></p>



<p>Please send in dry ice and immediately store all components in a -20℃ constant temperature refrigerator after receiving the reagent kit, which can be stored for 6 months. If you need to store it for a longer period of time, please store it below -70℃.</p>



<p><strong>Components</strong><strong></strong></p>



<figure class="wp-block-table"><table class="has-fixed-layout"><tbody><tr><td>Component</td><td>PCK244 24rxns</td><td>PCK244 96rxns</td></tr><tr><td>Cell Lysis Buffer</td><td>240μl</td><td>960μl</td></tr><tr><td>Cell Lysis Enzyme</td><td>16μl</td><td>64μl</td></tr><tr><td>Pre-Amp Buffer</td><td>120μl</td><td>480μl</td></tr><tr><td>Pre-Amp Enzyme Mix</td><td>7μl</td><td>28μl</td></tr><tr><td>Amplification Buffer</td><td>1.5ml</td><td>4×1.5ml</td></tr><tr><td>Amp Enzyme Mix</td><td>50μl</td><td>200μl</td></tr></tbody></table></figure>



<p><strong>Description</strong></p>



<p>The single-cell whole genome amplification kit can achieve whole genome amplification using single cells or trace samples as templates. The single-cell amplification reaction time is short, with a total process of about 3 hours. After lysis, pre amplification, and amplification, 2-5μg of genomic DNA can be obtained, with a size of around 200-1500bp. The amplified products can be widely used for second-generation sequencing, large segment copy number variation analysis, SNP typing, qPCR analysis, gene chip analysis, etc.</p>



<p><strong>Self provided instruments and reagents</strong></p>



<ol class="wp-block-list">
<li>PCR instrument</li>



<li>Reaction tube: It is recommended to use a low adsorption tube</li>



<li>Gun head: It is recommended to use high-quality filtering gun head</li>



<li>Micro centrifuge, vortex mixer</li>
</ol>



<p><strong>Note</strong></p>



<p>This product has extremely high detection sensitivity, and experimental operations should be completed in a positive pressure ultra clean workbench or clean environment. The concentration of amplification reaction products is high, and isolation should be done to avoid aerosol pollution caused by amplification products.</p>



<p><strong>Operation process diagram</strong></p>



<figure class="wp-block-image size-full"><img fetchpriority="high" decoding="async" width="1004" height="156" src="https://www.tinzyme.com/wp-content/uploads/image-47.png" alt="PCK244, Single Cell WGA Kit" class="wp-image-5298" srcset="https://www.tinzyme.com/wp-content/uploads/image-47.png 1004w, https://www.tinzyme.com/wp-content/uploads/image-47-300x47.png 300w, https://www.tinzyme.com/wp-content/uploads/image-47-768x119.png 768w, https://www.tinzyme.com/wp-content/uploads/image-47-260x40.png 260w, https://www.tinzyme.com/wp-content/uploads/image-47-50x8.png 50w, https://www.tinzyme.com/wp-content/uploads/image-47-150x23.png 150w" sizes="(max-width:767px) 480px, (max-width:1004px) 100vw, 1004px" /></figure>
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