Product Number:PCK54

Shipping and Storage

-30°C~ -15°C.Avoid repeated freeze-thaw cycles.

Component

Description

This product is a kit for one-step Real Time RT qPCR using RNA as a template using probe methods such as TaqMan and Molecular Beacon. When using this product for Real Time RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, without the need to add reagents or open the tube cap, which avoids contamination and improves experimental efficiency. This reagent introduces a dUTP/UG anti contamination system, which can rapidly degrade pollutants containing U at room temperature without affecting the efficiency and sensitivity of RT qPCR. This product is equipped with a freeze-drying protectant and can be used for the preparation of freeze-drying reagents.

Note

1. This product uses RNA as a template for one-step RT-PCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a dedicated area, using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with a 0.1% DEPC (diethyl carbonate) aqueous solution at 37 ℃ for 12 hours, and sterilized under high pressure for 30 minutes before use.
2. This product should avoid repeated freeze-thaw cycles and is recommended to be packaged and stored separately.
3. If there is precipitation or crystallization after melting the 5 × One Step Lyophilization Protector, please dissolve it in a 70 ℃ water bath without affecting its use.

Product Number: PCK63

Shipping and Storage

Store at 4-30°C, and store at -20°C until fully used after re dissolving. Avoid repeated freeze-thaw cycles as much as possible.

Components

Description

Lyo Universal One Step RT qPCR Mixture is a specialized in situ all component freeze-drying reagent that uses RNA as a template for quantitative PCR reactions. The buffer contains Taq DNA polymerase modified with novel antibodies, highly efficient thermally stable reverse transcriptase, enhancers and stabilizers.Mg²⁺ and dNTP are also present, and factors that effectively inhibit non-specific PCR amplification and enhance the efficiency of multiple qPCR reactions are added. This allows for multiple fluorescence quantitative amplification reactions while ensuring primer amplification efficiency. This product is convenient and fast to use, just add primer probes, and after extraction, the nucleic acid sample can be amplified on the machine. This product is compatible with both single and multiple probe qPCR reaction systems for routine and rapid detection.

Product Number: PCK49

Shipping and Storage

Stored at -30~-15℃, valid for 1 year. If frequent use is required, it can be packaged and stored at 2-8℃ to avoid repeated freeze-thaw as much as possible

Components

Description

This product is a specialized reagent kit for one-step RT-qPCR using probe methods (TaqMan, Molecular Beacon, etc.).When using this product for RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, and there is no need to add reagents or open the tube cap during the reaction process, which avoids pollution and improves experimental efficiency.This product has high detection sensitivity, strong fluorescence signal, and high signal-to-noise ratio, making it very suitable for the detection of trace amounts of RNA such as RNA viruses. The special buffer system contained in it can maximize the effectiveness of both reverse transcriptase and DNA polymerase, improving reaction efficiency.Using this product can obtain a wider linear range, more accurate quantification of the target gene, good repeatability, and high reliability. This kit introduces a dUTP-UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.UNG enzyme can rapidly degrade pollutants containing U at room temperature without affecting the formation of new dU based PCR products.

Notes

1. Before using the reagents in this kit, please gently mix them upside down to avoid foaming, and use them after brief centrifugation.
2. This product uses RNA as a template for one-step RT-qPCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a specialized area using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with 0.1% DEPC (diethyl pyrocarbonate) aqueous solution at 37℃ for 12 hours and sterilized under high pressure for 30 minutes before use.
3. The reagents in this kit should avoid repeated freezing and thawing; This product can be stored at -20℃ for long-term storage. If frequent use is required in the short term, it can be stored at 2-8℃.
4. This reagent kit must use specific primers, and the selection of primers can be based on specific experiments. The quality of primer design directly affects the results of RT-qPCR reaction. When designing primers, factors such as GC content, primer length, primer position, and the secondary structure of PCR products need to be considered. It is recommended to use professional primer design software for design.
5. It is recommended to use specific probes in this kit and use professional design software for design

Product Number:PCK27

Shipping and Storage

-30°C~ -15°C, transport ≤0°C.

Component

Description

Fast Probe One Step RT-qPCR, UNG Kit is designed specifically for quantitative PCR detection using RNA as a template, such as RNA viruses. Using gene-specific primers (GSPS), reverse transcription and qPCR reactions are performed in one tube without the need for additional tube opening/pipetting operations, greatly increasing assay throughput and reducing the risk of contamination. The dUTP/UNG anti-contamination system is introduced in this kit. Heat sensitive UNG can degrade U-containing pollutants rapidly at room temperature. Rapid inactivation of UNG by reverse transcription at 55°C did not affect the efficiency and sensitivity of qRT-PCR. Combined with optimized buffer system, antibody modified Taq enzyme and mutated M-MLV, the detection sensitivity of Fast Probe One Step RT-qPCR, UNG Kit can reach 0.1pg total RNA or <10 copies of RNA template and has higher thermal stability. 5×Fast Probe One Step RT-qPCR UNG Buffer contains optimized buffer system and dNTP/dUTP Mix, which is especially suitable for high specificity, low template concentration and multiple fast detection of fluorescent-labeled probes such as TaqMan.

Note

Before use, please gently mix the product upside down after it is completely melted, avoid foaming as far as possible, and use after a short centrifugation. Avoid freezing and thawing this product repeatedly. ROX dye is used to correct fluorescence signal errors generated between quantitative PCR holes. This product does not contain ROX dye.

Product Number: FER46

Shipping and Storage

Store at -20℃ and transport on dry ice.

Components

Description

Onestep DNA Frag And ER Reagent is a new generation of enzyme digestion repair reagents developed for Illumina and MGI high-throughput sequencing platforms. It can complete one-step fragmentation/final repair/addition of A. This product includes FER Enzyme Mix and FER Buffer, which can achieve fragmentation and end repair in one tube, avoiding cumbersome ultrasound processes and instrument dependence, and is easy to operate. It can achieve efficient and fast fragmentation, end repair, and dA tail addition for different DNA samples, and is applied to downstream library construction. It has the advantages of low preference, stable enzyme digestion repair effect, and higher library transformation efficiency. It can be applied to fragment, end repair, and A addition reactions of samples from different sources such as 0.1 ng-1ug genomic DNA, PCR amplification products, FFPE, etc.