Product Number: PCM355

Shipping and Storage

-20±5°C,try to avoid repeated freeze-thaw cycles.

Components

Description

Super Multiplex PCR Mix (UNG), Lyophilized is a premixed system suitable for various types of multiple PCR, with a concentration of 2.5×,Contains components such as DNA polymerase, UNG enzyme, PCR buffer, dNTPs, Mg²⁺, and enhancers.

Super Multiplex PCR Mix (UNG), a genetically modified recombinant enzyme containing DNA polymerase, has 5’→3′ DNA polymerase activity and no 5’→3′ exonuclease activity; DNA polymerase, modified by a new type of antibody, is an antibody modified hot start enzyme with high amplification efficiency. It also has excellent characteristics such as short activation time, strong amplification ability, and high sensitivity.This product introduces a dUTP/UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.

Super Multiplex PCR Mix (UNG) is suitable for anti pollution multiple PCR reactions, such as microsatellite analysis, genotyping, SNP detection, etc. This product can be paired with freeze-drying protectants for the preparation of freeze-drying reagents.

Notes

1. Before use, please gently mix the product upside down after it has completely melted, and centrifuge briefly before use.
2. Avoid repeated freeze-thaw of this product. Repeated freeze-thaw may cause a decrease in product performance, and it is recommended to store it separately.

Product Number: PCK49

Shipping and Storage

Stored at -30~-15℃, valid for 1 year. If frequent use is required, it can be packaged and stored at 2-8℃ to avoid repeated freeze-thaw as much as possible

Components

Description

This product is a specialized reagent kit for one-step RT-qPCR using probe methods (TaqMan, Molecular Beacon, etc.).When using this product for RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, and there is no need to add reagents or open the tube cap during the reaction process, which avoids pollution and improves experimental efficiency.This product has high detection sensitivity, strong fluorescence signal, and high signal-to-noise ratio, making it very suitable for the detection of trace amounts of RNA such as RNA viruses. The special buffer system contained in it can maximize the effectiveness of both reverse transcriptase and DNA polymerase, improving reaction efficiency.Using this product can obtain a wider linear range, more accurate quantification of the target gene, good repeatability, and high reliability. This kit introduces a dUTP-UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.UNG enzyme can rapidly degrade pollutants containing U at room temperature without affecting the formation of new dU based PCR products.

Notes

1. Before using the reagents in this kit, please gently mix them upside down to avoid foaming, and use them after brief centrifugation.
2. This product uses RNA as a template for one-step RT-qPCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a specialized area using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with 0.1% DEPC (diethyl pyrocarbonate) aqueous solution at 37℃ for 12 hours and sterilized under high pressure for 30 minutes before use.
3. The reagents in this kit should avoid repeated freezing and thawing; This product can be stored at -20℃ for long-term storage. If frequent use is required in the short term, it can be stored at 2-8℃.
4. This reagent kit must use specific primers, and the selection of primers can be based on specific experiments. The quality of primer design directly affects the results of RT-qPCR reaction. When designing primers, factors such as GC content, primer length, primer position, and the secondary structure of PCR products need to be considered. It is recommended to use professional primer design software for design.
5. It is recommended to use specific probes in this kit and use professional design software for design

Product Number: PCM14

Shipping and Storage

-20°C; try to avoid repeated freeze-thaw cycles.

Components

Description

Multiplex Pro MasterMix is a premixed system suitable for various types of multiple PCR, with a concentration of 2.5×, contains DNA polymerase, PCR buffer, dNTPs, Mg²⁺ and components such as stabilizers and enhancers, this product can be amplified by adding primers and templates. The operation is simple and convenient, reducing the probability of contamination, and improving detection flux and reproducibility.

The DNA polymerase contained in Multiplex Pro MasterMix is a genetically engineered recombinant enzyme with 5’→ 3′ DNA polymerase activity and no 5’→3′ exonuclease activity; DNA polymerase, modified by a new type of antibody, is an antibody modified hot start enzyme that can effectively reduce non-specific amplification generated by non-specific binding or dimerization of primers and templates at room temperature. It also has excellent characteristics such as short activation time, strong amplification ability, high sensitivity, and good stability.The unique combination of PCR buffer system and hot start enzyme significantly improves the amplification efficiency of PCR, with higher sensitivity and stronger inhibitor tolerance.

Multiplex Pro MasterMix has a wide range of applications and is suitable for various types of multiplex PCR, such as microsatellite analysis, amplification library construction, genotyping, and SNP detection.

Notes

1. Before use, please gently mix the product upside down after it has completely melted, and centrifuge briefly before use.
2. Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored at -20℃ for a long time.

Product Number: PCM34

Storage

-20°C, try to avoid repeated freezing and thawing.

Component

Description

2×Multiplex PCR MasterMix (UNG) is a PCR premix system composed of GoldStar Taq DNA Polymerase, Mg2+, dNTPs (including dUTP), UNG enzyme and PCR stabilizer. The use of this product does not require the optimization of complicated PCR reaction conditions, and multiple PCR reactions can be easily performed by simply exploring the conditions.

The GoldStar Taq DNA Polymerase contained in this product is a chemically modified hot-start enzyme, which can effectively reduce the non-specific amplification caused by primer mismatch in the early stage of PCR reaction. The unique buffer system enables all primers in the multiplex PCR reaction to be effectively extended without additional optimization. Also included in this MasterMix is the GC Enhancer, which facilitates efficient amplification of “difficult” templates (eg, those with high GC content). This product uses the dUTP-UNG anti-pollution system, which can effectively remove the residual pollution of PCR products and greatly reduce the false positives caused by the pollution of amplification products. The UNG enzyme can be inactivated by the pre-denaturation step in the PCR cycle, so it will not affect the formation of new dU-containing PCR products.

Multiplex PCR MasterMix (UNG) can effectively prevent residual contamination of PCR products, and is suitable for anti-pollution multiplex PCR reactions, such as microsatellite analysis, genotyping and SNP detection, etc.

Quality Control

After testing, there is no exogenous nuclease activity; PCR method detects no host residual DNA; storage at 2-8°C for 3 days, no significant change in amplification performance.