Product Number: PCM47

Storage Condition

-20±5℃.

Components

Description

SuperStar sProbe Mixture is a premix solution dedicated to real-time fluorescent quantitative PCR using probe methods (TaqMan, Molecular Beacon, etc.). The core component SuperStar DNA Polymerase is a hot-start DNA polymerase modified by double antibodies. It can recover DNA polymerase activity by heating at 95°C for 5 seconds. It has many advantages such as strong specificity and high detection sensitivity, and is equipped with the most suitable buffer optimized for qPCR. , the concentration of the reaction solution was 2×. The unique qPCR buffer system and hot-start enzyme combination of this product can effectively inhibit the generation of non-specific products and significantly improve the amplification efficiency of qPCR. It is very suitable for high specificity, high sensitivity, single and multiplex amplification, etc. qPCR reaction. Customers only need to add templates, primers, and probes, which is easy to use.

Pre-experiment preparation and precautions

• 1. Before use, please mix the product upside down and gently after it is completely melted, and use it after a short centrifugation.
• 2. This product is recommended to be used in small portions. If it needs to be used frequently in a short period of time, it can be stored at 2-8°C for 6 months.
• 3. Avoid repeated freezing and thawing of this product. Repeated freezing and thawing (freezing and thawing times ≤ 20 times) may reduce the performance of the product.
• 4. This product can be stored at -20±5°C and protected from light for a long time.
• 5. When configuring the reaction system of this product, it is best to carry out in a clean bench or a sterile environment.

Product Number: PCM63, PCM63L, PCM63H

Storage Condition

-20°C. For frequent use, it can be stored at 2-8°C. Try to avoid repeated freezing and thawing.

Components

Description

2×HotStar Probe Mix UNG is a premix system dedicated to real-time fluorescent quantitative PCR with a probe method (TaqMan, Molecular Beacon, etc.), with a concentration of 2×, including HotStar Taq DNA polymerase, PCR Buffer, dNTPs (dTTP is all replaced by dUTP replaced), UNG enzyme and Mg2+, the operation is simple and convenient. It is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription, such as gene expression analysis, copy number analysis, SNP genotype analysis, etc. The dUTP-UNG anti-pollution system is used in this product, and dUTP is added in the preparation process of the PCR reaction system, so an amplification product containing dU bases is formed. This product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction. In this way, the residual contamination of the PCR product is effectively removed, and the false positive caused by the contamination of the amplification product is greatly reduced. The UNG enzyme can be inactivated by the pre-denaturation step in the PCR cycle, so it will not affect the formation of new dU-containing PCR products. The HotStar Taq DNA Polymerase contained in this product is a chemically modified, brand-new high-efficiency hot-start enzyme, which has no polymerase activity at room temperature, and effectively avoids non-specific binding of primers and templates or primer dimers at room temperature. To generate non-specific amplification, enzyme activation must be incubated at 95°C for 10 minutes. The unique combination of PCR buffer system and hot-start enzyme significantly improves the amplification efficiency of PCR, with stronger fluorescent signal and higher sensitivity, and can detect single-copy templates. Using this product can get a wider linear range and more accurate quantification of the target gene.

ROX dye is used to correct the fluorescence signal error between the wells of the quantitative PCR instrument, and is generally used in Real Time PCR amplification instruments of companies such as ABI and Stratagene. The excitation optics of different instruments are different, so the concentration of ROX dye must be matched with the corresponding real-time PCR instrument.

Instruments that do not require ROX calibration (PCM63):

Roche LightCycler 480, Roche LightCyler 96, Bio-radi iCyler iQ, iQ5, CFX96, etc.

Instruments requiring Low ROX calibration (PCM63L):

ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 etc.

Instruments requiring High ROX calibration (PCM63H):

ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One/Step One Plus etc.

Note

1. Before use, please mix it upside down gently, try to avoid foaming, and use it after short centrifugation.

2. Avoid repeated freezing and thawing of this product, as repeated freezing and thawing may reduce product performance. Long-term storage of this product can be stored at -20°C, away from light. If it needs to be used frequently in a short period of time, it can be stored at 2-8°C.