Product Number: PCK48

Shipping and Storage

Box 1 -20℃ storage, dry ice transportation.

Box 2 2-8℃, transported in ice bags

Components

• Box 1

• Box 2

Description

The Universal DNA Library Kit (Illumina&MGI) is a second-generation sequencing enzyme digestion library kit developed for Illumina and MGI sequencing platforms. It includes three modules: DNA fragmentation/end repair/addition of A, adapter connection, and pre mixed enzymes required for library enrichment in library construction.By controlling the reaction time, sample DNA fragments from different sources such as 0.1ng-1μg genomic DNA, PCR amplification products, and FFPE can be reduced to small fragments, avoiding tedious ultrasound processes and instrument dependence.This kit simplifies the purification steps and shortens the library construction time. In the amplification module, high fidelity DNA polymerase is used for library enrichment, and PCR amplification is performed without preference, ensuring the accuracy of sequencing results.

Features

1. Accurate enzyme digestion, fragment end repair and A tube completion, without the need for purification and direct connection to the connector.
2. After connecting the joint, use matching magnetic beads for direct sorting (optional).
3. High fidelity enzymes for PCR enrichment and amplification minimize amplification preference.
4. Suitable for building libraries for different species such as humans, animals, plants, microorganisms, etc. The resulting library is applicable to various platforms such as Illumina and MGI.

Schematic diagram of DNA database construction process

DNA library construction process

Please read this operating instruction carefully before the experiment and choose the operating plan based on the type of sequencing platform used. Before the experiment starts, clarify the nucleic acid concentration, and the input amount of this reagent kit is 0.1ng-1μg. The recommended sample size is 1ng-500ng DNA. DNA solution does not contain chelating agents, if DNA dissolves in 1×TE or EDTA containing solutions, it is recommended to use magnetic beads for purification or add Neutralization Reagent.

Product Number: FER46

Shipping and Storage

Store at -20℃ and transport on dry ice.

Components

Description

Onestep DNA Frag And ER Reagent is a new generation of enzyme digestion repair reagents developed for Illumina and MGI high-throughput sequencing platforms. It can complete one-step fragmentation/final repair/addition of A. This product includes FER Enzyme Mix and FER Buffer, which can achieve fragmentation and end repair in one tube, avoiding cumbersome ultrasound processes and instrument dependence, and is easy to operate. It can achieve efficient and fast fragmentation, end repair, and dA tail addition for different DNA samples, and is applied to downstream library construction. It has the advantages of low preference, stable enzyme digestion repair effect, and higher library transformation efficiency. It can be applied to fragment, end repair, and A addition reactions of samples from different sources such as 0.1 ng-1ug genomic DNA, PCR amplification products, FFPE, etc.

Product Number:PCK13

Shipping and Storage

Box 1：Store at -20°C; transportation conditions: dried ice.

Box 2：Store at 2°C~8°C; transportation conditions: ice bag.

Components

Box 1: Cyclization reagents

Box 2: DNA Purification Beads

Description

Cyclation kit is a modular kit tailored for MGI’s high-throughput sequencing platform. This kit can be used to prepare PCR products from the spliced joint into single-stranded circular DNA libraries suitable for MGI sequencers. All reagents provided in the kit have undergone strict quality control and functional verification to ensure maximum stability and repeatability of library construction.

Consumables and Equipment

1. Magnetic rack: DynaMagTM-2 is recommended.
2. Qubit®3.0 ThermoFisher.
3. Qubit®3.0 ssDNA Assay Kit.
4. Anhydrous ethanol, EB (10mM Tris-HCl, pH 8.0), NF Water (pH 7.0 to 8.0) .
5. Reaction tube: It is recommended to use low adsorption PCR tube and EP tube.

Tips: It is recommended to use high quality filter gun head placement kit, library sample contamination

Preparation

1. Sample preparation
   1. PCR products: a total of 330 or more ng (multiple PCR product mix, refers to the total) after mixing, the volume of 49μL (if the PCR product itself insufficient volume, supplementary NF Water to total volume 49μL).
   1. PCR products fragment size: fragment peaks between 200-500bp.
   1. Modification: PCR products has joined for MGISEQ-2000, and BGISEQ MGISEQ -200-500 such as sequencing platform (Index) of fixed sequence.
2. Preparation of reagents
   1. Take out corresponding reagent kit, centrifugation, enzyme mixture on the ice to use: Before use, the buffer should be dissolved at room temperature, centrifuged by shock, placed on ice for use, NF Water and EB at room temperature for use.
   1. Please compound mixture on the ice.
   1. Please make reaction system on the ice.
   1. Kit buffer after dissolving in possible precipitation, precipitation does not affect the reagent function, please fully vibration blending to precipitation disappear after use.

Workflow

Product Number: PCK85M

Storage

Store at -20 ℃ and transport on dry ice.

Components

Description

This kit is suitable for both Illumina and MGI sequencing platforms. It provides the premixed enzyme modules required for DNA end repair,5′-end phosphorylation modification,3′-end A addition and Adaptor ligation in DNA library preparation. It can be used with different sequencing platform adapters. Primer kits can prepare DNA into specific DNA libraries for Illumina or MGI sequencing platforms. Using high-fidelity DNA polymerase for library enrichment and unbiased PCR amplification,the coverage area of the sequence is expanded,and high-quality DNA libraries can be prepared. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability of library preparation to the greatest extent.

Features

1. End repair and phosphorylation and adding A to complete in one step;
2. After the end repair without purification,directly add the Adaptor;
3. Ultra fidelity amplification,the maximum extent to reduce the amplification bias;
4. The library is suitable for multiple sequencing platforms:MGI sequencing platform:MGISEQ-2000, MGISEQ-200, BGISEQ-500 and other MGI sequencing platform lllumina GAllx, HiSacnSQ, HiSeq 250/2000/1000, MiSeqsequencing and other lllumina platform sequencers;
5. It is suitable for preparing gDNA and cDNA libraries after physical interruption.

Schematic diagram of DNA library construction process