Product Number: LG02

Shipping and Storage

Store at -30～-15 ℃ and transport at ≤ 0℃.

Components

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between adjacent 5′- phosphate and 3′ – hydroxyl ends on double stranded DNA or RNA. This enzyme can not only catalyze connections between flat or sticky ends, but also repair single strand cleavage in double stranded DNA, RNA, or DNA/RNA hybrid double strands.

Application

1. The connection between DNA fragments and carrier DNA.
2. The connection between DNA fragments and Linker or Adaptor DNA.

Unit definition

In a 20μl linkage reaction system, when 6μg of λDNA-Hind III decomposition product was reacted at 16℃ for 30 minutes, more than 50% of DNA fragments were linked, and the required enzyme quantity was defined as one active unit (U).

Product Number: LG0N

Shipping and Storage

Store at -20℃ and transport on dry ice.

Components

Description

T4 DNA Ligase is isolated and purified from Escherichia coli expressing the T4 DNA Ligase gene after induction and expression. It catalyzes the binding reaction of adjacent DNA strands with 5’phosphate groups and 3’hydroxyl groups via phosphodiester bonds. This enzyme can catalyze the connection of flat or sticky end DNA, repairing single stranded incisions in double stranded DNA, RNA, and DNA/RNA hybridization, but has no activity for single stranded nucleotides.

Application

Mainly used for adapter connection during the construction process of NGS Chinese library.

Unit definition

1U refers to the amount of enzyme required to convert 1nmol [32PPi] into Norit absorbable form within 20 minutes at 37°C in the ATP-PPi exchange reaction, equivalent to approximately 200 viscous terminal junction units.