Product Number: KL02

Shipping and Storage

-20°C

Components

Description

Klenow Fragment (3′-5′ exo -) is a mutated enzyme of DNA Klenow Fragment. This enzyme catalyzes the synthesis of complementary DNA to the template along the 3′-5′ direction using dNTP as a substrate in the presence of templates and primers. Through point mutation modification, this enzyme lost both the activity of the 3′-5′ exonuclease and the cleavage translation activity, while the point mutation modification made the 5′-3′ polymerase activity of this enzyme stronger. This product is a recombinant enzyme expressed in Escherichia coli, with a molecular weight of approximately 68.1 kDa. Enzymes have high specific activity, good stability, and strong compatibility with other enzymes.

Unit definition

The amount of enzyme required to add 10nmol dNTP to acid insoluble substances within 30 minutes at 37℃ is defined as 1 active unit (U).

Quality control

After multiple column purifications, SDS-PAGE detected a purity of over 99%; After testing, there was no contamination of nucleic acid endonucleases, exonucleases, phosphatases, and RNA enzyme activities.

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