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	<title>FER46 &#8211; Tinzyme</title>
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	<description>Enzymes, dNTP and rNTP</description>
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		<title>Onestep DNA Frag And ER Reagent</title>
		<link>https://www.tinzyme.com/ngs/onestep-dna-frag-and-er-reagent/</link>
		
		<dc:creator><![CDATA[tinzyme]]></dc:creator>
		<pubDate>Tue, 14 Jan 2025 01:18:48 +0000</pubDate>
				<category><![CDATA[NGS]]></category>
		<category><![CDATA[FER46]]></category>
		<category><![CDATA[Illumina]]></category>
		<category><![CDATA[MGI]]></category>
		<category><![CDATA[Onestep]]></category>
		<guid isPermaLink="false">https://www.tinzyme.com/?p=5358</guid>

					<description><![CDATA[FER46, Onestep DNA Frag And ER Reagent
Onestep DNA...]]></description>
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<p><a href="https://www.tinzyme.com/man/FER46.pdf" data-type="link" data-id="https://www.tinzyme.com/man/FER46.pdf" target="_blank" rel="noreferrer noopener">Manual</a></p>



<p><strong>Product Number: FER46</strong></p>



<p><strong>Shipping and Storage</strong></p>



<p>Store at -20℃ and transport on dry ice.</p>



<p><strong>Components</strong><strong></strong></p>



<figure class="wp-block-table"><table class="has-fixed-layout"><tbody><tr><td>Component</td><td>FER46</td><td>FER46</td></tr><tr><td>FER Buffer</td><td>240μl</td><td>960μl</td></tr><tr><td>FER Enzyme Mix</td><td>120μl</td><td>480μl</td></tr></tbody></table></figure>



<p><strong>Description</strong></p>



<p>Onestep DNA Frag And ER Reagent is a new generation of enzyme digestion repair reagents developed for Illumina and MGI high-throughput sequencing platforms. It can complete one-step fragmentation/final repair/addition of A. This product includes FER Enzyme Mix and FER Buffer, which can achieve fragmentation and end repair in one tube, avoiding cumbersome ultrasound processes and instrument dependence, and is easy to operate. It can achieve efficient and fast fragmentation, end repair, and dA tail addition for different DNA samples, and is applied to downstream library construction. It has the advantages of low preference, stable enzyme digestion repair effect, and higher library transformation efficiency. It can be applied to fragment, end repair, and A addition reactions of samples from different sources such as 0.1 ng-1ug genomic DNA, PCR amplification products, FFPE, etc.</p>
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