dNTP

Fluorescein-12-dUTP 1mM Sodium solution

Description

Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA, cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA, cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution. Fluorescein-12-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment and DNA Polymerase I.

Applications

Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

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dNTP

Biotin-11-dUTP 1mM Sodium solution

Description

Biotin-11-dUTP is enzymatically incorporated into DNA, cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA, cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose, magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.

Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5′-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.

Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptase, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.

Applications

Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension

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dNTP

Cy3-dUTP 1mM Sodium solution

Description

Cy3-dUTP can be used for direct enzymatic labeling of DNA, cDNA by PCR with Taq polymerase, Nick Translation with DNAse I, DNA Polymerase I, cDNA labeling and 3’-end labeling. Cy3-dUTP can replace TTP in reactions in which it serves as a substrate for T4 and Taq DNA polymerases, E. coli DNA polymerase (holoenzyme and Klenow fragment), reverse transcriptase (from AMV and MMLV) and terminal transferase. The synthesized dye-labeled DNA, cDNA probes can be used for the identification of specific sequences by in-situ hybridization, microarray or blotting techniques. Cy3-dUTP can also be used for multicolor fluorescence labeling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Cy3-dUTP, dTTP ratio for PCR and Nick Translation: 30-50% Cy3-dUTP, 50% dTTP.

Features

• High sensitivity.
• Low nonspecific binding.
• High photostability.
• High water solubility.
• pH insensitive.

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Description

• Product Name: 2′-Deoxyuridine-5′-triphosphate, sodium salt solution
• Molecular Formula: C₉H₁₂N₂Na₃O₁₄P₃
• Molecular Weight: 534.1
• CAS Number: 102814-08-4

Quality control

1. The concentration is verified by optical density spectrophotometry.
2. This preparation is free of DNase and RNase contamination.
3. The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.
4. This lot of dUTP has been performance tested with Taq DNA polymerase and Pfu DNA polymerase.
5. This lot of dUTP has performance tested with Q-PCR.

Storage Recommendations

Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Always avoid freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.

Standard package size

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available

Delivery Time

2 working days

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Description

This dNTP/dUTP mix are manufactured by MEBEP Bio Science, one of a few primary manufacturers in the industry.

The dNTP/dUTP Mix 10mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dUTP, each at 10mM in water.
So the total concentration of nucleotide therefore is 40mM. For direct use in PCR with Taq DNA Polymerase, reverse transcription and other applications.

Quality Control

Functionally tested in PCR with Taq DNA Polymerase.
Greater than 99% purity of each component confirmed by HPLC.
Free of human and E. coli DNA.
Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.

Applications

For use in all molecular biology applications including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA and DNA sequencing.

Note

dNTP/dUTP mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

Storage

Store at -20°C.

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available, OEM and Bulk both are available

Delivery Time

2 working days

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Description

• Product Name: 2′-Fluoro-2′-deoxyuridine-5′-triphosphate, lithium salt solution
• Molecular Formula: C₉H₁₄FN₂O₁₄P₃ (free acid)
• Molecular Weight: 486.1 (free acid)
• Appearance: Clear colorless solution
• Concentration: 100mM(±5%)
• Purity(HPLC): ≥95%
• 31P-NMR Conforms to the structure
• λmax: (pH 7.0) 262nm±2nm
• pH: (22~25°C) 7.0±0.1

Quality Control Molecular Biology Grade

This lot of dNTP has been performance tested in transcription reaction.
This preparation is free of DNase and RNase contamination.

Storage Recommendation

Long term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C
Avoid repeated freeze-thaw cycles or exposure to frequent temperature changes.
These fluctuations can greatly alter product stability.
Upon first use, it is recommended to aliquot sample into single use portions.
Retest Date 1 year

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MOQ: 1ml of each
Delivery time: 2-3 working days after receive the payment.
Payment method: T/T or western Union or Money Gram full in advance.
OEM and Bulk both are available

The 2’F-dNTP(2′-Fluorine-dNTP) we supplied is the 100mM soulution of 2’F-dATP, 2’F-dGTP, 2’F-dCTP, and 2’F-dUTP in sepreate tubes.